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Study of Squalamine Lactate for the Treatment of Macular Edema Related to Retinal Vein Occlusion

Phase 1
Completed
Conditions
Retinal Vein Occlusion
Macular Edema
Interventions
Registration Number
NCT02614937
Lead Sponsor
Ohr Pharmaceutical Inc.
Brief Summary

This was a prospective, single center, open label, randomized study evaluating the biological effect of squalamine lactate ophthalmic solution, 0.2% combined with intravitreous ranibizumab in patients with macular edema secondary to branch, hemi-central and central retinal vein occlusion (BRVO, HRVO, CRVO).

Detailed Description

At baseline, all eyes underwent ETDRS visual acuity measurements at 4 meters, a complete ophthalmological evaluation, SD-OCT imaging of the macula, and fluorescein angiographic assessment of capillary perfusion in the macula and peripheral fundus. All eyes received an initial 10 week mandatory loading period of topical squalamine therapy.

All eyes received mandatory intravitreal injections of ranibizumab 0.5mg at the conclusions of weeks 2 and 6. At the conclusion of week 10, eyes were randomized in a 1:1 ratio to continue squalamine drops bid or discontinue squalamine drops in the study eye. All eyes were examined every 4 weeks through the week 38 endpoint and were eligible to receive additional as needed ranibizumab 0.5mg injections starting at the conclusion of week 10 and every 4 weeks thereafter through week 34 depending upon prespecified visual acuity and OCT retreatment criteria.

Any eye with a decrease of 5 or more ETDRS letters or increase in CST on OCT of 50uM or more from their best previous measurements automatically received an additional ranibizumab 0.5mg injection beginning at the conclusion of week 10.

Eyes randomized to continue squalamine drops did so through the week 38 endpoint. SD-OCT measurements of the macula were obtained at every study visit. Fluorescein angiograms were performed on the study eye at baseline, weeks 10 and 38.

Safety endpoints included all adverse events spontaneously reported, elicited or observed were documented by the investigators at any visit.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
20
Inclusion Criteria
  • Eyes with treatment naïve, center involving macular edema secondary to BRVO, HRVO or CRVO in patients of at least 40 years of age
  • Macular edema of 1-4 months duration prior to the baseline visit
  • Best corrected baseline ETDRS visual acuity of 20/40 to 20/320 Snellen equivalent using the 4 meter testing method
  • Baseline CST greater than or equal to 325uM using SD-OCT imaging
  • Less than 50% foveal capillary ring disruption as defined by fluorescein angiography (FA)
  • Absence of dense intraretinal or subretinal hemorrhage and or lipid through the foveal center
  • Absence of subfoveal fibrosis or hyperpigmentation.
Exclusion Criteria
  • Eyes with ocular pathology other than RVO related macular edema such as clinically significant cataract or media opacity, diabetic retinopathy, macular degeneration, glaucoma, uveitis, epiretinal membrane, vitreomacular traction or intraocular tumor
  • Intraocular surgery within 6 months prior to baseline
  • Two-plus or greater afferent pupillary defect (APD) in the study eye
  • Likelihood of evidence driven indication for peripheral scatter photocoagulation within 6 months of recruitment
  • History of previous intravitreal pharmacologic treatment of any kind in the study eye
  • History of previous retinal laser photocoagulation of any kind in the study eye
  • History of intravitreal anti-VEGF therapy in the fellow eye within 6 months prior to baseline
  • Any evidence of baseline ocular neovascularization such as disc neovascularization, preretinal neovascularization, iris or angle neovascularization in the study eye
  • Eyes that have shown spontaneous improvement within the preceding 3 months defined as an improvement of best corrected visual acuity of greater than 15 ETDRS letters or thinning of the CST on OCT of greater than 20%

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Squalamine and ranibizumab to Week 10Squalamine Lactate Ophthalmic Solution, 0.2%All eyes received an initial 10 week mandatory loading period of topical Squalamine Lactate Ophthalmic Solution, 0.2% therapy. All eyes received mandatory intravitreal injections of ranibizumab 0.5mg at the conclusions of weeks 2 and 6. Randomize at Week 10 to 2 different groups - Squalamine and No Squalamine, continue PRN ranibizumab in both groups
Stop Squalamine, ranibizumab PRNSqualamine Lactate Ophthalmic Solution, 0.2%Discontinue use of Squalamine Lactate Ophthalmic Solution, 0.2% after Week 10; continue ranibizumab 0.5 mg IVT PRN
Continue Squalamine, ranibizumab PRNranibizumabContinue use of Squalamine Lactate Ophthalmic Solution, 0.2% after Week 10; continue ranibizumab 0.5 mg IVT PRN
Squalamine and ranibizumab to Week 10ranibizumabAll eyes received an initial 10 week mandatory loading period of topical Squalamine Lactate Ophthalmic Solution, 0.2% therapy. All eyes received mandatory intravitreal injections of ranibizumab 0.5mg at the conclusions of weeks 2 and 6. Randomize at Week 10 to 2 different groups - Squalamine and No Squalamine, continue PRN ranibizumab in both groups
Stop Squalamine, ranibizumab PRNranibizumabDiscontinue use of Squalamine Lactate Ophthalmic Solution, 0.2% after Week 10; continue ranibizumab 0.5 mg IVT PRN
Primary Outcome Measures
NameTimeMethod
Visual Function - EfficacyBaseline to Week 38

Mean change in ETDRS letter score from baseline

Secondary Outcome Measures
NameTimeMethod
Visual Function - EfficacyBaseline to Week 38

Eyes with visual outcomes of 20/40 or better at Week 38 compared to Baseline Visit

Retinal Anatomy - EfficacyBaseline to Week 38

Proportion of eyes with Central Subfield Thickness (CST) on SD-OCT less than 325 microns at Week 38

Safety and Tolerability as measured by adverse event reporting and ophthalmologic examination from Baseline to Week 38Baseline to Week 38

Safety as measured by adverse event reporting and ophthalmologic examination from Baseline to Week 38

Concomitant ranibizumab administration - efficacyBaseline to Week 38

Number of injections of 0.5 mg ranibizumab to keep edema resolved from Week 2 through Week 34 of study

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