CS-206 in Patients With Sickle Cell Disease

Early Phase 1

Recruiting

• Conditions: Sickle Cell Disease
• Interventions: Genetic: CS-101

• Registration Number: NCT06565026
• Lead Sponsor: CorrectSequence Therapeutics Co., Ltd

Brief Summary

The goal of this open label, single-arm clinical study is to learn about the safety and efficacy of CS-101 injection in treating sickle cell disease.

Detailed Description

CS-101 is an autologous CD34+ cell suspension, edited by in vitro base editing technology, which modifies the BCL11A binding site in HBG promoter, so that it loses the ability to bind to BCL11A, which can re-induce the production of γ-globin chain and increase the concentration of fetal hemoglobin(HbF) in the blood, compensating for the function of missing adult hemoglobin HbA to achieve clinical cure. The therapy addresses two major challenges in the current treatment of the disease: lack of matching donors and graft-versus-host diseases in allogeneic hematopoietic stem cell transplantation.

Study Timeline

• Study First Submitted: 2024-08-13
• Study First Submitted QC: 2024-08-19
• Study First Posted: 2024-08-21
• Study Start: 2024-09-02
• Status Verified: 2024-11
• Last Update Submitted: 2024-11-07
• Last Update Posted: 2024-11-11
• Primary Completion: 2026-12-31
• Study Completion: 2027-06-30

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 5

Inclusion Criteria

• Participants must be between 12 to 35 years old (inclusive). Participants or their legal guardians (for participants below 18 years old) must provide written informed consent before any study-related procedures.

• Participants must have a Documented βS/βS, βS/β0 or βS/β+ genotype.

• Participants must have at least one of the following conditions

  1. At least 2 occurrences of any of the following events within 2 years prior to screening.

     1. Acute pain crisis: requiring a visit to a medical facility and administration of pain medications (opioids or intravenous NSAIDs) or red blood cell transfusions.
     2. Acute chest syndrome: defined by the presence of a new pulmonary infiltrate on a chest X-ray, associated with pneumonia-like symptoms, including chest pain, fever, or respiratory distress.
     3. Priapism lasting more than 2 hours and necessitating a visit to a medical facility for intervention.
     4. Stroke or transient ischemic attack (TIA): confirmed by imaging studies (e.g., MRI or CT scan), including silent stroke, and overt stroke leading to neurological deficits lasting >24 hours.

  2. Presence of red cell alloimmunization (>2 antibodies) and the need for ongoing chronic transfusions.

  3. Participants who have failed, not tolerated, refused the standard of care for Sickle Cell Disease (SCD), or are unable to access the standard of care due to the availability

  4. Other situations deemed appropriate for hematopoietic stem cell transplantation according to the sickle cell anemia treatment guidelines, as determined by the investigator.

• Laboratory Parameters:

  1. Documented Hemoglobin S (HbS) level ≥30% of total hemoglobin (Hb) concentration prior to transfusion.
  2. HbF at screening < 20%

• Participants must have a Karnofsky Performance Status (KPS for participants above 16 years old, inclusive) or Lansky Play-Performance Scale (LPPS for participants below 16 years old) score of ≥70, indicating sufficient functional status to undergo the intervention.

• Willing to comply with the protocol requirements, use contraception as required, attend regular follow-up visits, and cooperate with examinations.

Exclusion Criteria

• Female participants who are pregnant, breastfeeding, or planning pregnancy during the study period are excluded.

• Participation in another investigational drug trial within 30 days prior to screening or within 5 half-lives (whichever is longer).

• Subjects who have received or are receiving luspatercept treatment within 3 months prior to screening.

• Subjects who have previously received any gene therapy for the disease.

• Subjects with a fully matched related donor who are already scheduled for allogeneic hematopoietic stem cell transplantation.

• More than 10 unplanned hospitalizations or emergency visits within 12 months prior to screening, which the investigator believes are related to significant chronic pain rather than acute pain crisis (VOC).

• Severe liver dysfunction:

  1. Aspartate aminotransferase (AST) or alanine aminotransferase (ALT) >3× the upper limit of normal (ULN) or:
  2. International Normalized Ratio (INR) >1.5× ULN

• Severe renal impairment (creatinine clearance <30 mL/min/1.73 m²) are excluded.

• Subjects with HIV, cytomegalovirus (CMV), Epstein-Barr virus (EBV), or Treponema pallidum infection during the screening period; those with active HBV or HCV infection; or known tuberculosis or parasitic infection, etc. Excludes subjects with stable hepatitis B (HBV-DNA negative) after treatment and those cured of hepatitis C (HCV-RNA negative). Known active bacterial, viral, or fungal infections.

• Deemed unsuitable for autologous hematopoietic stem cell transplantation procedures as determined by the investigator.

• Other situations deemed unsuitable for this study as determined by the investigator.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
CS-101	CS-101	Autologous CD34+ hematopoietic stem cell suspension modified by in vitro base editing technique

Primary Outcome Measures

Name	Time	Method
AEs(Adverse Events) and SAEs(Serious Adverse Events) after CS-101 infusion	From signing informed consent to 24 months post-CS-206 infusion	Frequency and severity of adverse events(AEs）as assessed by CTCAE(Common Terminology Criteria for Adverse Events)v5.0
Incidence of transplant-related mortality	From baseline to 100 days and 12 months post-CS-206 infusion	Incidence of transplant-related mortality(Transplant-related mortality events defined as deaths assessed by the investigator as potentially transplant-related)
Time to neutrophil engraftment	Up to 24 months post-CS-206 infusion	Time to neutrophil engraftment is defined as first day of 3 consecutive measurements of absolute neutrophil count≥0.5×10\^9/L on three different days.
Time to platelet engraftment	Up to 24 months post-CS-206 infusion	Time to platelet engraftment is defined as first day of 3 consecutive measurements of absolute platelet count≥20×10\^9/L on three different days and without platelet transfusion.
All-cause mortality	Up to 24 months post-CS-206 infusion
Free from severe VOCs for 12 consecutive months (VF12)	starting 60 days after the last red blood cell transfusion up to 24 months	Free from severe vaso-occlusive crises (VOCs) for 12 consecutive months (VF12)

Secondary Outcome Measures

Name	Time	Method
Free from hospitalization due to severe vaso-occlusive crises for 12 consecutive months(HF12)	starting 60 days after the last red blood cell transfusion up to 24 months
Free from severe VOCs for 9 consecutive months (VF9)	starting 60 days after the last red blood cell transfusion up to 24 months	Free from severe vaso-occlusive crises (VOCs) for 9 consecutive months (VF9)
Annualized incidence of severe vaso-occlusive crises (VOC)	starting 60 days after the last red blood cell transfusion up to 24 months
Annualized incidence of hospitalization due to severe vaso-occlusive crises	starting 60 days after the last red blood cell transfusion
HbF (fetal hemoglobin) level in blood samples	up to 24 months post-CS-206 infusion
Proportion of edited alleles in peripheral blood leukocytes and bone marrow cells, and persistence and chimerism kinetics evaluation	up to 24 months post-CS-206 infusion

Trial Locations

Locations (1)

The First Affiliated Hospital of Guangxi Medical University; 🇨🇳 Nanning, Guangxi, China