Evaluation of Biomarker Levels in Gingival Crevicular Fluid of Patients With Different Periodontal Diseases
- Conditions
- Periodontal Diseases
- Interventions
- Diagnostic Test: GCF obtaining
- Registration Number
- NCT04777487
- Lead Sponsor
- Izmir Katip Celebi University
- Brief Summary
Objectives: The aim of this study is; detection of peptidyl arginine deiminase4 (PAD4), galectin-3 and tumor necrosis factor alpha (TNF-α) levels in gingival crevicular fluid (GCF) samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples of GCF were obtained from 60systemically healthy non-smoker individuals with periodontitis (P, n=20), gingivitis(G, n=20) and healthy periodontium (S, n=20). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine PAD4, galectin-3 and TNF-α levels in the biological samples.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 60
- Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.)
- At least twenty permanent teeth in the mouth
- Non-smoker
- No medication for continuous use
- Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
- Not in pregnancy or lactation period.
- For the periodontitis group that has not received periodontal treatment in the last 6 months
- For the periodontitis group; According to the evaluation made in 6 regions of each tooth, individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss
- For the gingivitis group; According to the evaluation made in 6 regions of each tooth, individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss
- For healthy group; According to the evaluation made in 6 regions of each tooth,individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.
- Any oral or systemic disease
- Regularly using a systemic medication
- During pregnancy or lactation
- Received periodontal treatment within the last 6 months.
- Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
- Smokers are not included in the study
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Gingivitis GCF obtaining Full-mouth clinical periodontal measurements recorded and GCF obtained. Healthy Periodontium GCF obtaining Full-mouth clinical periodontal measurements recorded and GCF obtained. Periodontitis GCF obtaining Full-mouth clinical periodontal measurements recorded and GCF obtained.
- Primary Outcome Measures
Name Time Method The total amount of Galectin-3 in GCF 24 hours after taking the clinical measurements at the first visit The total amount of Galectin-3 in GCF
The total amount of PAD4 in GCF 24 hours after taking the clinical measurements at the first visit The total amount of PAD4 in GCF
- Secondary Outcome Measures
Name Time Method The total amount of TNF-α in GCF 24 hours after taking the clinical measurements at the first visit The total amount of TNF-α in GCF
Trial Locations
- Locations (1)
Izmir Katip Çelebi University Department of Periodontology
🇹🇷İzmir, Turkey