The Significance of Intact Liver Innervation for the Glucose and GLP-1 Induced Insulin Secretion
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- insulin secretion
研究概览
简要总结
The aim of the study is to investigate the significance of intact nerve supply to the liver for the glucagon-like peptide-1 (GLP-1) induced insulin secretion.
The hypothesis is that the effects of GLP-1 is transmitted through the GLP-1 receptor and that these effects involve sensory afferent neurons, probably primarily parasympathetic.
详细描述
GLP-1 is a potent enterogastron and incretin hormone. It is rapidly inactivated by dipeptidyl peptidase IV so only 10-15% enters the systemic circulation. This has led to the hypothesis that GLP-1 interact locally with afferent sensory nerve fibers.
The aim of this study is to investigate the significance of intact liver innervation for the GLP-1 induced insulin secretion in liver transplanted patients; kidney transplanted control patients matched for immunosuppressive treatment, age, gender and body weight; and ten control persons matched for age, gender and body weight.
The insulin secretion will be evaluated from blood samples that will be analyzed for insulin and c-peptide.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •normal fasting plasma glucose
- •normal hemoglobin
- •informed consent
排除标准
- •type 1 diabetes mellitus or type 2 diabetes mellitus
- •body mass index > 30
- •inflammatory bowel disease
- •intestinal surgery
- •serum creatinine > 250 µM and/or albuminuria
- •ALAT > 2 x normal value
- •Severe cardiac insufficiency
- •in treatment with medicine which cannot be paused for 12 hours
研究组 & 干预措施
Liver transplanted
10 Liver transplanted patients
干预措施: dipeptidyl peptidase 4 (DPP4) inhibitor (Drug)
Liver transplanted
10 Liver transplanted patients
干预措施: oral glucose (Other)
Liver transplanted
10 Liver transplanted patients
干预措施: intravenous glucose (Other)
Kidney transplanted
10 kidney transplanted individuals
干预措施: dipeptidyl peptidase 4 (DPP4) inhibitor (Drug)
Kidney transplanted
10 kidney transplanted individuals
干预措施: oral glucose (Other)
Kidney transplanted
10 kidney transplanted individuals
干预措施: intravenous glucose (Other)
Healthy controls
10 healthy controls
干预措施: dipeptidyl peptidase 4 (DPP4) inhibitor (Drug)
Healthy controls
10 healthy controls
干预措施: oral glucose (Other)
Healthy controls
10 healthy controls
干预措施: intravenous glucose (Other)
结局指标
主要结局
insulin secretion
时间窗: four hours
The insulin secretion during a four-hour oral glucose tolerance test (OGTT) and an intravenous isoglycaemic clamp is evaluated
次要结局
- plasma glucagon(12 time points within four hours)
- plasma glucose(20 time points within four hours)
- plasma GLP-1(12 time points within four hours)
- plasma GIP(12 time points within four hours)
- plasma GLP-2(12 time points within four hours)
- plasma PYY(12 time points within four hours)
研究者
Jonatan I Bagger
MD
University Hospital, Gentofte, Copenhagen