Intact Vagal Innervation and Glucagon-like Peptide-1 (GLP-1) Effects
- Registration Number
- NCT01176890
- Lead Sponsor
- University Hospital, Gentofte, Copenhagen
- Brief Summary
The aim of this study is to investigate the role of transmission via the vagal nerve for the effect of Glucagon-like peptide-1 (GLP-1) in respect to gastric emptying, appetite and food intake.
The hypothesis is that a great deal of the effects of GLP-1 is mediated via the nervous system and for this reason the researchers will investigate individuals with and without intact nervous supply.
- Detailed Description
GLP-1 is a potent enterogastron and incretin hormone. It is rapidly inactivated by dipeptidyl peptidase IV so only 10-15% enters the systemic circulation. This has led to the hypothesis that GLP-1 interact locally with afferent sensory nerve fibers. We investigated the role of intact vagal innervations on the effect of GLP-1 on the food intake, gastric emptying (GE) and appetite.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 30
- truncal vagotomy
- normal hemoglobin
- informed consent
- Age, gender and weight matched controls
- normal hemoglobin
- informed consent
- type 1 diabetes mellitus or type 2 diabetes mellitus
- body mass index > 30 kg/m2
- inflammatory bowel disease
- intestinal resection
- nephropathy (serum creatinine > 150 µM and/or albuminuria)
- liver disease (ALAT and/or ASAT > 2 x normal value)
- treatment with medicine which cannot be paused for 12 hours
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Vago GLP-1 Truncally vagotomized subjects (due to duodenal ulcer operation) Vago Saline Truncally vagotomized subjects (due to duodenal ulcer operation) Cardia Saline truncally vagotomized subjects (due to esophagus resection) Healthy controls GLP-1 Healthy control subjects Healthy controls Saline Healthy control subjects Cardia GLP-1 truncally vagotomized subjects (due to esophagus resection)
- Primary Outcome Measures
Name Time Method Gastric emptying four hours Gastric emptying will be assessed using a liquid meal with 1.5 g paracetamol added and continuous blood sampling for measuring paracetamol concentration during four hours
Appetite four hours Hunger, satiation, and fullness will be assessed before, during and after a liquid meal with 1.5 g paracetamol added using a visual analog scale (VAS)
Food intake five hours After the liquid meal test, the volunteers will be offered a meal to assessed their ad libitum food intake
- Secondary Outcome Measures
Name Time Method plasma glucose four hours serum insulin and c-peptide four hours plasma glucagon four hours plasma GLP-1 four hours endogenous GLP-1 four hours endogenous GLP-1 will be assessed as plasma GLP-2 concentration
plasma GIP four hours serum paracetamol four hours
Trial Locations
- Locations (1)
Department of Internal Medicine F' laboratory
🇩🇰Hellerup, Copenhagen, Denmark
Department of Internal Medicine F' laboratory🇩🇰Hellerup, Copenhagen, Denmark