The impact of pH on fertilisation in human oocytes

Not Applicable

Recruiting

• Conditions: IVF; Infertility; Reproductive Health and Childbirth - Fertility including in vitro fertilisation

• Registration Number: ACTRN12617000230358
• Lead Sponsor: Westmead Fertility Centre

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2017-02-13
• Last Update Posted: 10 December 2019

Recruitment & Eligibility

• Status: Recruiting
• Sex: Female
• Target Recruitment: 300

Inclusion Criteria

Patients will be asked if they would like to participate if they present for fertility treatment at WFC and fulfil the following:
1) Good understanding/ability of English language to enable them to give an informed consent
2) Less than 42 years old at the time of her treatment cycle
3) Equal to or greater than 8 oocytes collected
4) No more than one prior failed fresh cycle at WFC
5) Heterosexual and same sex couples will be offered to take part

Exclusion Criteria

Patients will be excluded from this study if;
1) There is severe male factor infertility requiring sperm extraction via microdissection/open biopsy (PESA/TESA patients can be included)
2) A prior history of poor fertilisation (less than 50 percent oocytes fertilising normally)
3) Oncology patients
4) Unable to speak English without assistance of an interpreter

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
umber of eggs fertilised in solution A (control group) will be compared with the number of eggs fertilised in solution B (treatment group). Fertilisation will be compared by positively identifying the presence of two pronuclei inside the egg the day after it has been exposed to sperm (either via in vitro fertilisation or intra-cytoplasmic sperm injection).[16-19hrs post exposure to partner sperm]

Secondary Outcome Measures

Name	Time	Method
The fertilised eggs will be kept in the lab for upto 5 days for pre-implantation development before they are transferred back to the patients uterus. <br>The eggs which were fertilised in solution A (control) will be kept separately from the eggs fertilised in solution B (treatment group) throughout the pre-implantation development period. Embryo grading will be performed on all embryos on the day of transfer (as standard) this could be day 2, 3 or 5 of embryo development. Grades will be recorded per embryo to allow for retrospective analysis to understand if pH at the time of fertilisation has an impact on embryo quality.[Embryo grades are awarded on the day of embryo transfer - this may be either 2, 3 or 5 days after fertilisation has taken place (routine practice). The grade will be recorded in the patient notes and then transferred to our secure database.<br>Embryo grading is carried out using the Gardner Scoring System, which is used universally to score embryos.<br>]