Adipose Tissue Cellular Response to Exercise in Healthy Humans
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- University of Michigan
- Enrollment
- 40
- Locations
- 1
- Primary Endpoint
- Quantifying the change in markers of macrophage abundance in adipose tissue
Study Overview
Brief Summary
The purpose of this study is to measure the effects of a single session of exercise on changes in the cellular composition of the body fat of humans.
Detailed Description
Fat tissue is far more than just a place where humans store body fat. Fat tissue contains many different types of cells, such as immune cells, endothelial cells, and fat precursor cells. Differences or changes in the number of immune cells, endothelial cells, and fat precursor cells in fat tissue can have a great impact on metabolic health. The abundance of all immune cells, endothelial cells, and fat precursor cell types are known to change when a person loses or gains weight. For example, the abundance of inflammatory cells is often found to decrease markedly with weight loss - and this change has been linked to the profound improvements in metabolic health that occur with weight loss. In contrast, the effects of exercise on immune cells, endothelial cells, and fat precursor cells in fat tissue is not known. Determining the effects of exercise on changes in the cellular composition within fat tissue will provide important information for optimizing lifestyle interventions aimed at improving metabolic health.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 40 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Age: 18-40
- •Body Mass Index: 20-27 kg/m2
- •Regular exercise (less than or equal to 3 days/week of aerobic exercise; 30-60 minutes/session at moderate and vigorous intensities)
- •Women must have regularly occurring menses and must be premenopausal
Exclusion Criteria
- •Blood pressure greater than 140/90 mm Hg
- •Pregnant or lactating
- •Evidence/history of cardiovascular or metabolic disease
- •Medications known to affect lipid or glucose metabolism, or inflammation
Arms & Interventions
Trial
Subjects will need to come to the Substrate Metabolism Laboratory in the morning around 7:00 AM. After about a 15-30 minute rest, the study team will collect a blood sample from the subject's hand or forearm. The study team will also obtain a small sample of fat tissue from the area just underneath the skin near the belly button. Next, subjects will exercise on a treadmill at a moderate intensity for about 1 hour. Immediately after exercising the study team will collect a blood sample. The subject will remain resting in the laboratory for 3 hours after exercise, and then the study team will collect another blood and fat tissue sample. Upon completion, the subject will be provided a snack before leaving the laboratory.
Intervention: Exercise (Other)
Outcomes
Primary Outcomes
Quantifying the change in markers of macrophage abundance in adipose tissue
Time Frame: 1 day
The stromovascular fraction will be isolated from subcutaneous lipo-aspirate samples by collagenase digestion and a series of filtration/wash steps (routinely performed by our lab). This isolation protocol has been optimized to yield the minimum number of stromal cells required to identify and recover the following cell populations by fluorescence-activated cell sorting (FACS), listed as cell surface stain = cell type: CD64+/CD11c+ = macrophages FACS will be performed at the University of Michigan Flow Cytometry Core, and sorted cells will be recovered for subsequent gene expression analysis using the StepOnePlus real time PCR system (Life Technologies) with fluorescent probe-based assays.
Secondary Outcomes
- Quantifying the change in markers of endothelial cell and pre-adipocytes in adipose tissue(1 day)
Investigators
Jeffrey F Horowitz
Associate Professor, Movement Science, School of Kinesiology
University of Michigan