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Exploring the effect of post-exercise blood biomarkers in healthy volunteers on skin cancer cell lines.

Not Applicable
Conditions
Basal Cell Carcinoma
Cutaneous squamous cell carcinoma
Cancer - Non melanoma skin cancer
Registration Number
ACTRN12624000966594
Lead Sponsor
niversity of Canberra
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
ot yet recruiting
Sex
All
Target Recruitment
6
Inclusion Criteria

Participants will be recreationally active, healthy adults, 25-40 years old.

Exclusion Criteria

Participants must not have any prior or current history of or being
treated for any chronic illness or cancer, including any type of skin cancer. All participants with current or previous history of chronic
illness or cancer including cancer will be excluded from the study

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Effect of exercised serum on cell viability on keratinocyte cancer cell lines[Cell proliferation assay 3 repeated trials (to assess for replicability), 4 weeks in between each trial. Assessed at each of the 3 repeated trials conducted at week 1, 4 and 8.<br>];Effect of exercised serum on apoptosis on keratinocyte cancer cell lines[Apopercentage assay 3 repeated trials (to assess for replicability), 4 weeks in between each trial. Assessed at each of the 3 repeated trials conducted at week 1, 4 and 8.]
Secondary Outcome Measures
NameTimeMethod
Characterizing changes in immune biomarkers (Interleukin-6, ) after exercise[Enzyme-linked immunosorbent assays (ELISA kits) 3 repeated trials (to assess for replicability), 4 weeks in between each trial. Assessed at each of the 3 repeated trials conducted at week 1, 4 and 8.];Characterizing changes in immune markers (Natural Killer Cells, T cells activation and proliferation)[IL-15 Enzyme-linked immunosorbent assays (ELISA kits) 3 repeated trials (to assess for replicability), 4 weeks in between each trial. Assessed at each of the 3 repeated trials conducted at week 1, 4 and 8.]
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