Effect of CoQ10 on the Endocrine Function of Skeletal Muscle

Not Applicable

Not yet recruiting

• Conditions: Coenzyme Q Deficiency; Exercise Training; Ischemic Preconditioning
• Interventions: Dietary Supplement: CoQ10 supplementation; Dietary Supplement: Sham IPC combined with placebo; Dietary Supplement: IPC training combined with CoQ10 supplementation; Procedure: Ischemic preconditioning training

• Registration Number: NCT05412888
• Lead Sponsor: Medical University of Gdansk

Brief Summary

Recent studies have also shown that repeated episodes of ischemia, followed by reperfusion (IPC), can contribute to the development of adaptive changes not only in the area of the heart muscle, but also in the structure of the skeletal muscles.

In the project, several research questions will be evaluated e.g. what is the relationship between oxidative stress parameters, uric acid concentration and nitric oxide degradation products in groups of people undergoing two-week training in ischemic training, or what is the relationship between the expression of genes associated with muscle cell growth (e.g. myostatin gene) and the effect of ischemia preconditioning training etc.

Detailed Description

Both in sport and medicine, methods are constantly sought that can significantly contribute to improving the ability of tissues to perform their functions and protecting them from effort-induced damage.

In a wide range of interests, especially in people treated for ischemic changes of cardiovascular diseases, are protocols of tissue preconditioning by transient ischemia which significantly contribute to the increase in blood flow in tissue structure.

Recent studies have also shown that repeated episodes of ischemia, followed by reperfusion (IPC), can contribute to the development of adaptive changes not only in the area of the heart muscle, but also in the structure of the skeletal muscles. There is still a lack of detailed research on genetic conditions related to adaptive changes in this area of interest.

In the project, the following research questions were posed:

1. What is the relationship between oxidative stress parameters, uric acid concentration and nitric oxide degradation products in groups of people undergoing two-week training in IPC training, or 2 weeks of CoQ10 supplementation in the light of non-training and non-supplementation?

2. What is the relationship between two weeks of lower limb ischemia preconditioning training, or CoQ10 supplementation, and the expression of genes associated with the stress response (HSF-1, NF-kB, TNF dependent glass)?

3. What is the relationship between the expression of genes associated with muscle cell growth (e.g. myostatin gene) and the effect of preconditioning training by ischemia?

4. What is the relationship between the expression of genes associated with intracellular metabolism and the effect of CoQ10 supplementation?

5. What is the relationship between the expression of genes encoding enzymes of energy pathways (e.g. GAPDH, LDH) and IPC training or CoQ10 supplementation in the study group?

6. How does IPC training or CoQ10 supplementation affect the expression of genes responsible for membrane transport, and the Hedgehog pathway in muscle cells (including Gli1)?

7. How does IPC training affect the expression of transcription factors: Hif-1-alpha (hypoxia-induced factor) and NF-kB and selected genes dependent on their activity?

HYPOTHESIS The level of gene expression associated with oxidative stress, muscle fiber development, angiogenesis, muscle cell energy, and membrane transport will change under the influence of the use of preconditioning training by deficiency or CoQ10 supplementation

Study Timeline

• Study First Submitted: 2022-05-25
• Study First Submitted QC: 2022-06-07
• Study First Posted: 2022-06-09
• Status Verified: 2023-01
• Last Update Submitted: 2023-01-02
• Last Update Posted: 2023-01-04
• Study Start: 2023-03-01
• Primary Completion: 2023-07-31
• Study Completion: 2026-09-30

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: Male
• Target Recruitment: 100

Inclusion Criteria

• gender (males only),

• not taking medication during the study,
• good health (no concomitant injuries),
• negative history of cardiovascular disorders, autonomic nervous system, mental disorders, cerebrospinal injuries and other diseases that may directly affect the results obtained.

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Exclusion Criteria

Lack of consent to participate in the research project or negative result of medical examinations.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Supplemented population 300mg	CoQ10 supplementation	Supplemented population 300mg/24h CoQ10 - 25 participants
Control group (placebo)	Sham IPC combined with placebo	Control group to CoQ10 supplementation (placebo)- 25 participants
IPC control group	IPC training combined with CoQ10 supplementation	IPC control group - 25 participants
Training and IPC preconditioning	Ischemic preconditioning training	Group subjected to training and preconditioning by ischemia (IPC training) - 25 participants,

Primary Outcome Measures

Name	Time	Method
The effects of IPC on physical performance	8 weeks	IPC training will be combined with endurance exercise and VO2max and serum Lactate will be measured
The effects of CoQ10 supplementation on physical performance	8 weeks	CoQ10 supplementation combined with endurance training will be evaluated for endurance improvement in young men. VO2max, myokines, genes associated with the stress response will be measured
The effects of CoQ10 supplementation and IPC on physical performance	8 weeks	CoQ10 supplementation combined with IPC and endurance training will be evaluated for endurance improvement in young men. VO2max, myokines, genes associated with the stress response, ulic acid will be measured

Trial Locations

Locations (1)

Jedrzej Antosiewicz; 🇵🇱 Gdańsk, Pomorskie, Poland