GCF miRNA-223 , -214 Levels in Smokers and Nonsmokers Periodontitis Patients

Completed

• Conditions: Periodontitis, Adult
• Interventions: Diagnostic Test: miRNA-223 & -214 detection

• Registration Number: NCT06064799
• Lead Sponsor: Fayoum University

Brief Summary

Objective: Periodontitis is a multifactorial disease that affects a wide range of populations. However, its pathogenesis remains unclear. miRNAs are now considered potential diagnostic markers for many inflammatory diseases. Thus, the aim of this study was to assess the expression of microRNA-223(miRNA-223) and microRNA-214 (miRNA-214) in gingival crevicular fluid (GCF) of smoker and nonsmoker with periodontitis.

Materials and Methods: We conducted a prospective study among 42 participants: 14 healthy controls, 14 nonsmoker periodontitis participants, and 14 smoker with periodontitis. Eligibility criteria for inclusion were consecutive adults, aged 20-60 years, with stage III periodontitis grade B/C and no systemic diseases. All consenting participants had gingival crevicular fluid samples collected after diagnosis to assess miRNA-214 and -223 by quantitative real-time polymerase chain reaction assay.

Detailed Description

Not available

Study Timeline

• Study Start: 2023-01-01
• Primary Completion: 2023-06-30
• Study Completion: 2023-06-30
• Status Verified: 2023-09
• Study First Submitted: 2023-09-20
• Study First Submitted QC: 2023-09-26
• Last Update Submitted: 2023-09-26
• Study First Posted: 2023-10-03
• Last Update Posted: 2023-10-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 42

Inclusion Criteria

• both genders
• age from 20 to 60 years
• no more than 4 teeth lost due to periodontitis
• systemically free participants

Exclusion Criteria

• Pregnant or lactating female
• participants with a history of an inflammatory or immunological disease
• history of any current medication

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Group I	miRNA-223 & -214 detection	14 healthy volunteers
Group III	miRNA-223 & -214 detection	14 periodontitis participants with stage III grade C Periodontitis
Group II	miRNA-223 & -214 detection	14 periodontitis participants with stage III grade B Periodontitis

Primary Outcome Measures

Name	Time	Method
assessment of miRNA-223 & -214 levels in GCF samples in all groups	level of miR-223 & -214 are measured at baseline after the completion of all GCF sample collection carried at the day of enrollment of subjects in the investigation after diagnosis confirmation	PCR kits are used for assessment of miRNA-223 \& -214 levels in GCF samples in all groups

Trial Locations

Locations (1)

faculty of dentistry, Fayoum university; 🇪🇬 Fayoum, Egypt