Mushroom Ingestion Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 7
- 试验地点
- 2
- 主要终点
- Plasma ergothioneine
研究概览
简要总结
The investigators propose to determine the effect of consuming 0, 1 or 2 servings of white button (Agaricus bisporus; most popular, lower ergothioneine) or yellow oyster (Pleurotus citrinopileatus; higher ergothioneine) mushrooms on postprandial changes in plasma levels and urinary excretions of mushroom-specific compounds.
详细描述
The investigators hypothesize that consuming different mushroom varieties will elicit distinct postprandial nutrimetabolomic profiles in the plasma and urine and that the amount of metabolites present will increase with the servings of mushrooms. Measuring ergothioneine in the plasma will provide a quantitative outcome to document the postprandial responses to consuming the test meals. The nutrimetabolomic related outcomes are exploratory and have not been documented in human research.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Other
- 盲法
- Single (Investigator)
入排标准
- 年龄范围
- 30 Years 至 69 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Male or female;
- •age 30-69 y;
- •BMI: 25.0-34.9 kg/m2;
- •Not severely or extremely depressed (Beck's Depression Inventory score ≤30)
- •Total cholesterol <240 mg/dL, low-density lipoprotein cholesterol <160 mg/dL, triglycerides <400 mg/dL, fasting glucose <110 mg/dL;
- •Systolic/diastolic blood pressure <140/90 mm Hg;
- •Body weight stable for 3 months prior (± 3 kg);
- •Stable physical activity regimen 3 months prior;
- •Medication use stable for 6 months prior;
- •Non-smoking;
- •Non-diabetic;
- •Not acutely ill;
- •Females not pregnant or lactating;
- •Participants must be willing and able to consume the prescribed diets and travel to testing facilities.
排除标准
- •BMI <25 or >35;
- •Severely depressed (Beck's Depression Inventory score >30);
- •Total cholesterol >240 mg/dL, low-density lipoprotein cholesterol >160 mg/dL, triglycerides >400 mg/dL, fasting glucose >110 mg/dL;
- •Body weight changes in previous 3 months (±3 kg);
- •Changes in physical activity regimen in the previous 3 months;
- •Medication changes in the previous 6 months;
- •Diabetic;
- •Acute illness;
- •Pregnant or lactating;
- •Allergic to mushrooms
结局指标
主要结局
Plasma ergothioneine
时间窗: 5 hours
Plasma will be assessed for ergothioneine concentration, a compound unique to mushrooms
Plasma triglycerides
时间窗: 5 hours
Plasma will be assessed for triglyceride values at the time of salad consumption and at each hour for 5 hours after salad consumption
Change in plasma insulin
时间窗: 5 hours
Plasma will be assessed for insulin values prior to salad consumption and in response to the test day meal
Change in plasma glucose
时间窗: 5 hours
Plasma will be assessed for glucose values prior to salad consumption and in response to the test day meal
Plasma metabolomics
时间窗: 5 hours
Plasma will be assessed for metabolomics (i.e. ergothioneine) via reverse phase liquid chromatography/mass spectrometry
次要结局
未报告次要终点
研究者
Wayne Campbell
Principal Investigator
Purdue University