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Intravenous Iron Effects on Performance at High Altitude

Phase 4
Recruiting
Conditions
Hypoxia
Interventions
Other: Placebo
Registration Number
NCT06686693
Lead Sponsor
United States Army Research Institute of Environmental Medicine
Brief Summary

To compare the effects of IV iron versus placebo (saline) injection on arterial oxygen saturation, submaximal exercise responses, and 2-mile treadmill time-trial performance during acute exposure to hypobaric hypoxia (430 mmHg, simulating \~4800m) assessed 1 and 14 days after treatment.

Primary Hypothesis 1: IV iron treatment will improve arterial oxygen saturation at rest and during exercise in acute hypobaric hypoxia and this effect will persist for 2 weeks

Primary Hypothesis 2) IV iron treatment will improve 2-mile treadmill time trial performance in acute hypobaric hypoxia and this effect will persist for 2 weeks

Detailed Description

This study will use a double-blind randomized study design in which one group will receive iron (n = 12; Injectafer, 15 mg/kg up to 1000 mg) and the other a placebo (n = 12; saline). Twenty-four participants will complete seven study visits: Visit 1: Orientation visit consisting of dual-energy X-ray absorptiometry scan (DEXA), pulmonary function test, assessment of peak aerobic capacity (VO2peak); Visits 2-3: familiarization consisting of exercise battery (20 minutes of walking followed by 2 mile treadmill time trial); Visits 4, 6-7: high altitude trial (4,800 m) in which skin blood flow during rest will be measured, ventilation at sea level and altitude will be assessed, collection of sea level and altitude arterialized capillary and venous blood will be collected, and the exercise battery. Visit 5: participants will receive the iron or placebo.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
24
Inclusion Criteria
  • Man or woman, age 18-40 years

    • BMI 18.5-30 kg/m2
    • In good health as determined by the Office of Medical Support & Oversight (OMSO) General Medical Clearance
    • Passed his/her most recent Army Combat Fitness Test (ACFT) (Military Volunteers Only) or exercises at least 2 times per week and able to complete two mile run in ≤ 21 minutes (Civilian Volunteers)
    • Willing to not exercise, or drink alcoholic/caffeinated beverages 24 hours prior to each testing session
    • Willing to not perform any strenuous exercise 36 hours prior to each testing session
Exclusion Criteria
  • Women who are pregnant or planning to become pregnant during the study

    • Taking prescription medication, or over-the-counter medications other than contraceptives, unless approved by OMSO & PI
    • Taking dietary supplements unless approved by OMSO & PI
    • Born at altitudes greater than 2,100 m (7,000 ft)
    • Living in areas that are more than 1,200 m (4,000 ft) or have traveled to areas that are more than 1,200 m for five days or more within the last 2 months
    • Prior diagnosis of High Altitude Pulmonary Edema (HAPE) or High Altitude Cerebral Edema (HACE)
    • Musculoskeletal injuries that compromise the ability to walk/run on a treadmill
    • Presence of abnormal blood chemicals (hemoglobin S or Sickle Cell Trait)
    • Hemoglobin concentration: men: Hb <13.5 g/dL or Hb >17.7 g/dL; women Hb <12.5 g/dL or Hb >15.9 g/dL
    • Ferritin < 50 ng/mL or greater than 150 ng/mL
    • Any history of malignancy
    • Personal or family history of blood clots
    • History of thromboembolic disease, hypertension, and known risk factors of cardiovascular disease
    • Blood donation within 8 weeks of beginning the study
    • History of seizures
    • History of inflammatory bowel disease
    • Any recent (within 4-6 weeks) and or expected history of prolonged periods of immobility or limited activity (including recent or upcoming surgery)
    • Abnormal PT/PTT test or problems with blood clotting
    • Any nicotine or recreational drug use (unless quit > 1 month prior to study orientation)
    • Presence of respiratory tract infections (< 1 month prior)
    • Experience recent cold, coughs, or sinus infections (< 2 weeks prior)
    • Allergy to skin adhesive
    • Evidence of apnea or sleeping disorder
    • Present condition of alcoholism, use of anabolic steroids, other substance abuse issues
    • Body mass <50 kg (110.5 lbs)
    • History of hyperparathyroidism
    • History of vitamin D deficiency
    • History of systemic inflammatory disease (rheumatoid arthritis, lupus erythematosus)
    • Any drug allergies
    • History of hypersensitivity reaction
    • History of asthma
    • History of kidney or liver disease
    • Any previous intravenous iron injection

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboPlacebo - saline
Intravenous ironInjectaferIron - Injectafer 15 mg/kg up to 1000 mg
Primary Outcome Measures
NameTimeMethod
2 mile treadmill time trial performanceBaseline at sea level, pre intervention at altitude, one week post intervention at altitude, and two weeks post intervention at altitude.

Time to complete self-paced 2 miles treadmill time trial (min:sec)

Secondary Outcome Measures
NameTimeMethod
Ventilation ratePre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Ventilation rate will be assessed using a pneumotachometer (l/min).

Percent body fatWill be assessed once at the beginning of the study.

Percent body fat will be assessed using Dual Energy X-ray Absorptiometry (%body fat).

Oxygen SaturationPre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Oxygen saturation will be assessed using a finger pulse oximeter (%).

HematocritPre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Hematocrit will be assessed using manual hematocrit method (%).

FerritinPre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Ferritin will be assessed using an ELISA (ng/ml).

C-reactive proteinPre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

C-reactive protein will be assessed using an ELISA (mg/L).

Soluble transferrinPre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Soluble transferrin will be assessed using an ELISA (mg/L).

Arterialized capillary PO2Pre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Arterialized capillary PO2 will be assessed using a blood gas analyzer (mmHg).

Arterialized capillary PCO2Pre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Arterialized capillary PCO2 will be assessed using a blood gas analyzer (mmHg).

Arterialized capillary pHPre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Arterialized capillary pH will be assessed using a blood gas analyzer.

Skin Blood flowPre intervention at sea level and altitude, one week post intervention at sea level and altitude, and two weeks post intervention at sea level and altitude.

Skin blood flow will be assessed via laser doppler (arbitrary units).

VO2peakWill be assessed once on day 1.

Peak aerobic capacity will be assessed using a metabolic cart (ml/kg/min and or l/min).

Pulmonary function testWill be measured once on day 1.

Pulmonary function test will be performed to determine forced expired volume in one second (FEV1; L) using a metabolic cart.

Trial Locations

Locations (1)

USARIEM

🇺🇸

Natick, Massachusetts, United States

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