Circulating endothelial cells in sickle cell disease

Recruiting

Conditions: Sickle cell
Sickle cell anemia
10018902

Registration Number: NL-OMON30432

Lead Sponsor: Erasmus MC, Universitair Medisch Centrum Rotterdam

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: Not specified

Target Recruitment: 15

Inclusion Criteria

1. High performance liquid chromatography confirmed diagnosis of HbSS or HbSb0-thalassemia genotype.
2. Written informed consent by the patient.
3. For healthy controls: HbAA genotype, race sex and age matched blood donors, written informed consent.

Exclusion Criteria

1. Blood transfusion in the preceding three months.
2. Painful crisis/acute chest syndrome/stroke or other acute complications in the preceding 2 weeks.
3. Pregnancy, active cancer, active infection, connective tissue diseases.
4. For healthy controls: any current disease

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1. Comparison of the number of CEC in sickle cell patients to the number in<br /><br>healthy controls.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>2. To study the relationship of CEC to the history of clinical vaso-occlusion<br /><br>(e.g., the frequency of painful crises).<br /><br>3. To analyze the relationship of CEC to established organ damage and<br /><br>dysfunction.<br /><br>4. To determine the relationship of CEC to laboratory risk factors for poor<br /><br>outcome (e.g., the degree of anemia and the percentage of fetal haemoglobin).<br /><br>5. To analyze the relationship of CEC to other biomarkers of disease activity<br /><br>(e.g. endothelial activation and fucntion, the hypercoagulable state and<br /><br>angiogenesis.</p><br>