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Determination of intestinal microbiota in faecal specimens and intestinal mucosa biopsy specimens using bacterial DNA-based profiling methods.

Completed
Conditions
gut bacteria
10017969
obesitas
intestinal microbiota
Registration Number
NL-OMON39196
Lead Sponsor
Vrije Universiteit Medisch Centrum
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
Not specified
Target Recruitment
350
Inclusion Criteria

Age 18 years and older
Informed Consent
Elective colonoscopy
Patient living within a 15km radius from the VUmc
Pre-endoscopy colonic cleansing with Kleanprep®

Exclusion Criteria

Contraindication for colonoscopy
Contraindication for harvesting biopsy specimens
Other pre-endoscopy colonic cleansing than with Kleanprep®

Study & Design

Study Type
Observational invasive
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
<p>Primary outcome is a bacterial profile of the intestinal microbiota derived<br /><br>from the inter spacer region PCR (IS-PCR) technique. </p><br>
Secondary Outcome Measures
NameTimeMethod
<p>Faecal and mucosal profiles will be compared together, and with other DNA based<br /><br>techniques. </p><br>
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