Cytokine Expression of IL-8 Before and After Root Canal Treatment in Patients With Symptomatic Apical Periodontitis: Clinical Trial
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 36
- 试验地点
- 1
- 主要终点
- interleukin 8
研究概览
简要总结
This clinical study aimed to Compare the levels of Interleukin 8 before and after root canal treatment in patients with apical periodontitis
- Diagnosis
- Local anesthesia
- Isolation and disinfection of the tooth
- Two-staged access cavity preparation
- Patency of the root canals
- First sample collection using paper points
- Working length determination
- Chemico-mechanical preparation
- After 1 week, isolation, Second sample collection then obturation of the root canals and restoration of the tooth.
- Samples will be stored in (-80 C) freezer till collection of all samples then, quantification will be done using ELISA.
详细描述
Diagnosis:
- Personal data including name, address and phone number in addition to medical history and dental history will be collected from each patient in specific forms, then history of chief complaint will be recorded. The selected patients should show symptoms of symptomatic apical periodontitis (SAP) either associated with vital or necrotic teeth.
- Extra-oral and intra-oral clinical examination of the suspected tooth will be performed tentatively using a diagnostic mirror and probe.
- Radiograph will be taken using bisecting angle technique with a periapical digital sensor.
- The diagnosis of symptomatic apical periodontitis is confirmed through positive percussion test.
- After completing the diagnosis, the patient will be enrolled in the study.
Treatment procedure:
-
Local anesthesia: Anesthetizing the tooth using infiltration technique by local anesthesia (4% Articiane).
-
Isolation and disinfection: Operative field, including the tooth, the clamp, and rubber dam sheet will be disinfected using 30% hydrogen peroxide followed by 5% sodium hypochlorite solution. Subsequently, 5% sodium thiosulfate will be used to inactivate the disinfecting agents.
-
Access cavity preparation: a 2-stage access cavity preparation will be performed. The first stage involves the removal of caries and/or coronal restorations using sterile diamond bur. In the second stage, before entering the pulp chamber, the cavity will be disinfected according to the previous decontamination protocol. Then, a new round bur and tapered stone with round end will be used for access cavity preparation.
-
Patency of the root canal will be confirmed using stainless steel hand K-files size #10 and #15.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Screening
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Medically free patients
- •Single canaled teeth diagnosed clinically with symptomatic apical periodontitis.
- •Radiographic widening in the periodontal membrane space.
- •Presence of pain on percussion.
- •Patients' acceptance to participate in the trial.
排除标准
- •Medically compromised patients.
- •Pregnant women: Avoid radiation exposure, anesthesia, and medication.
- •If analgesics or antibiotics have been administrated by the patient during the past 24 hours preoperatively.
- •Teeth that shows association with acute periapical abscess or swelling: Need special treatment steps which could involve additional visits with incision and drainage.
- •Presence of periodontal disease
- •Non restorability
- •Immature teeth
- •Radiographic evidence of external or internal root resorption vertical root fracture, perforation, calcification.
研究组 & 干预措施
Vital teeth
root canal instrumentation sodium hypochlorite 2.5% irrigation
干预措施: Root canal instrumentation, 2.5% NaOCl irrigation (Procedure)
Non-vital teeth
root canal instrumentation sodium hypochlorite 2.5% irrigation
干预措施: Root canal instrumentation, 2.5% NaOCl irrigation (Procedure)
结局指标
主要结局
interleukin 8
时间窗: immediately after access cavity preparation and immediately after root canal instrumentation
Cytokine expression of IL8
次要结局
未报告次要终点
研究者
Sarah Abouelenien
Lecturer of Endodontics
Cairo University