n-3 Fatty Acid Effects in T2DM After Acute High Intensity Exercise
- Conditions
- Type2 Diabetes Mellitus
- Interventions
- Dietary Supplement: Placebo GroupDietary Supplement: n-3 PUFA Group
- Registration Number
- NCT03182712
- Lead Sponsor
- Federal University of Rio Grande do Sul
- Brief Summary
The aim of this study was to verify the effect of a combined supplementation \[n-3 PUFA (known to induce anti-inflammatory effects) plus vitamin E (known for its antioxidant properties)\] on lipidic, glycemic, redox status and inflammatory parameters in type 2 diabetic patients. In addition, considering that exercise, at high intensities, can induce a transient inflammatory state and increase oxidative stress (OS) markers, the investigators tested if our nutritional intervention could attenuate this response within this population (thus, using high intensity exercise as inductor of acute inflammatory/OS state).
- Detailed Description
Herein the investigators tested the effect of eight weeks of omega-3 polyunsaturated fatty acid (n-3 PUFA) and vitamin E supplementation before and after an acute high intensity exercise bout on lipid profile, glycemic, redox and inflammatory parameters in type 2 diabetic (T2DM) patients. Thirty T2DM patients, without chronic complications, took part in this study. Blood and urine samples were collected after 12 h-fasting state for baseline biochemical analysis. Thereafter, subjects performed an incremental workload VO2max test on a cycle ergometer to determine the load of the high intensity submaximal exercise. On the following week, blood samples were collected before and immediately after the exercise test for measurements of oxidative stress (OS) and high-sensitivity C-reactive protein (hs-CRP). Afterwards, participants were randomly allocated into two groups: placebo (gelatin) and n-3 PUFA (capsules containing 180 mg of eicosapentaenoic acid, 120 mg of docosahexaenoic acid and 2 mg of vitamin E). Three capsules were ingested daily for eight weeks. Then, the examination protocol and the exercise test were repeated and the samples collected.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 30
- Clinical diagnosis of Type 2 Diabetes
- Male and female
- Age between 40 and 60 years old.
- Must be able to do exercise
- Smoking;
- Clinical diagnosis of kidney, pulmonary or heart diseases
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Placebo Group Placebo Group Subjects receiving gelatin capsules (500mg). Three capsules were ingested daily for eight weeks. n-3 PUFA Group n-3 PUFA Group Subjects receiving n-3 PUFA (capsules containing 180 mg of eicosapentaenoic acid, 120 mg of docosahexaenoic acid and 2 mg of vitamin E). Three capsules were ingested daily for eight weeks.
- Primary Outcome Measures
Name Time Method Changes on hs-CRP (High-sensitivity C reactive protein) Measured before the supplementation (baseline) and after eight weeks of supplementation Marker of inflammation expressed in mg/dL
- Secondary Outcome Measures
Name Time Method Changes on F2-isoprostanes Measured before the supplementation (baseline) and after eight weeks of supplementation Marker of oxidative stress expressed in ng/mL
Changes on Total Antioxidant activity Measured before the supplementation (baseline) and after eight weeks of supplementation Marker of oxidative stress expressed in contains per minute
Changes on thiobarbituric acid reactivity Measured before the supplementation (baseline) and after eight weeks of supplementation Marker of oxidative stress expressed in nmol/mL
Trial Locations
- Locations (1)
Clinical Hospital of Porto Alegre
🇧🇷Pôrto Alegre, RS, Brazil