Genome-Wide Association Study With the Aim of Implantable Cardioverter Defibrillator Implantation Genophenotypic Risk Stratification

Not yet recruiting

• Conditions: Arrythmia; Sudden Cardiac Death; ICD
• Interventions: Other: Non-Invasive saliva sampling

• Registration Number: NCT05582252
• Lead Sponsor: Selcuk University

Brief Summary

This clinical study will be conducted with funding from European Innovation Council(EIC) after approval of the fund grant and is part of our organization's European Pathfinder Project(Ref: HORIZON-EIC-2022-PATHFINDERCHALLENGES-01). The clinical study step of this project will be started in a retrospective time prospective manner by gathering the phenotypic(clinical measuring factors) data from patients who underwent ICD implantation therapy. The study will be done as a case-control type in which patients who did not get any shocks in 6 months post-implantation will be allocated to the control group. A customized and highly specific cardiogenomics panel will be designed and ordered to be specially manufactured as a standard kit by Illumina® (San Diego, California, U.S.) following an exhaustive investigation for collecting genetic variants which correlated to cardiovascular development. Mentioned kit bears the standard and validated technology which is part of the genetic tests routine and is being produced by Illumina® incorporate. However, as an option manufacturer is designing custom kits for research purposes by getting the desired variant lists using the same technology. Accordingly, enrolled patients in the study will be prospectively sampled ( Non-Invasive saliva sampling) for getting genetically analysed by Illumina®'s Infinium Assay Microarray platform with fully customized 700,000 single nucleotide polymorphism kits. The result of this sampling will be data and statistically analysed in a genome-wide association study(GWAS) manner by considering the 5x10-8 p.value and will be associated with each phenotypic parameter. Accordingly, the study will assess the genetic risk stratification in ICD patients in a much more detailed fashion. Following this assessment genophenotypic statistical analysed will be done to combine both parameters and generate a formula for scoring the indicator factors based on each odds ratio. Correspondingly, this new scaling formula will be analysed, verified and validated further by a randomized sampling of the population in our study before being stated. Additionally, This study will not only help to improve current genetic polymorphism clinal significant status (pathogenicity and significance of variant) but also can associate new markers with high significance that can be directly used in clinical screening, diagnosis or clinical approaches.

Detailed Description

Not available

Basic Information
|
Recruitment & Eligibility
|
Study & Design
|
Trial Locations

Study Timeline

• Status Verified: 2022-10
• Study First Submitted: 2022-10-12
• Study First Submitted QC: 2022-10-12
• Last Update Submitted: 2022-10-12
• Study First Posted: 2022-10-17
• Last Update Posted: 2022-10-17
• Study Start: 2023-02
• Primary Completion: 2024-06
• Study Completion: 2025-03

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 2500

Inclusion Criteria

• ICD-implanted patients (both for primary and secondary intervention)
• Being a volunteer for the study
• Adequacy in understanding the study risks and accepting the Informed Consent Form
• Official acceptance of the legal and official parents (both father and mother), If younger than 18 years old

Read More

Exclusion Criteria

• The patient who does not volunteer to involve to the study.
• Diagnosis of underlying arrhythmogenic disease (Structural Heart disease, Brugada, Arrhythmogenic right ventricular dysplasia, etc.)
• Development of electric shock due to acute coronary syndrome
• Atrial Fibrillation (AFib) With Rapid Ventricular Response
• Electric shock in patients with electrolyte imbalance-induced VT/VF
• Electric Shock in a patient with acute myocarditis-induced ventricular arrhythmias
• Patients that had an electric shock because of pacing / ATP ramp-induced VT (RV/CRT Pacing)

Read More

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Control Group	Non-Invasive saliva sampling	Patients with ICD who didnot received any electric shock in 6 months after ICD implantation
Healthy Group	Non-Invasive saliva sampling	Healthy Volunteers This group will be used for related bioinformatic analysis (DATA analysis stage) but not in statistical analysis
Experimental group	Non-Invasive saliva sampling	Patients with ICD who received at least one electric shock in 6 months after ICD implantation

Primary Outcome Measures

Name	Time	Method
Revealing SNP variants related to phenotypic measurement factors	2024MAY	ICD Shock, Sudden Cardiac Death, Ventricular Arrythmia and ICD prognosis related genetic polymorphism variant
Comprehensive genophenotypic scoring system formula	2025MARCH	a formula that is based on genetic and phenotypic parameters that can be used as criteria for ICD implantation indication
GENOPHENOTYPIC risk stratification	2024DEC	Results of genotype and phenotype statistical analysis

Secondary Outcome Measures

Name	Time	Method
offering a comprehensive cardiogenetic kit	2025MARCH	a kit that is based on the Illumina bead chip Infinium Microarray technology and can be used for research, clinical screening and diagnosis

Trial Locations

Locations (1)

Selcuk University Genetic Department Faculty of Medicine; 🇹🇷 Konya, Turkey