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Comparison Between Biopsied and Non-biopsied Intracytoplasmatic Sperm Injection (ICSI) Embryos and Natural Pregnancy Embryos

Recruiting
Conditions
Women Conceiving by: 1- ICSI-PGS 2- ICSI-non-PGS 3- Spontaneously Conceiving Women
Interventions
Procedure: Biopsied ICSI embryos (PGS)
Registration Number
NCT04280757
Lead Sponsor
Wael Elbanna Clinic
Brief Summary

As Pre Implantation Genetic Screening (PGS) becomes now a replacement modality and trend gaining popularity among physicians and patients on claiming its ability to settle on the simplest genetically normal embryo, so it had been logical to review if taking cells from the embryo in its early development will or won't affect its growth as compared to the previous standard technique of ICSI and in comparison to a normally conceived embryos the study also will question if the parameters of the prenatal biochemical standard parameters will have any differences if the embryo is already genetically tested embryo and compare these parameters with non PGS embryos and normally conceived embryos.

This study is an ambispective, comparative, cohort, observational, single-center study.

The study participants' relevant medical records will be collected and reviewed for the retrospective subjects and after obtaining informed consent for the prospective subjects. The study materials that will be used will include blood tests and ultrasound. The study will involve three study arms

Detailed Description

This study is an ambispective, comparative, cohort, observational, single-center study that will be conducted at Wael ElBanna Clinic, a private center that is well equipped for all procedures needed for ICSI and fetal medicine. After obtaining informed consent, the study participants' relevant medical records will be collected and reviewed. Data will be collected from the subjects' medical records. Data will be pooled and presented in aggregate, without identification of individual subjects.

The study materials that will be used will include blood tests and ultrasound. The study will involve three study arms:

* Arm 1: biopsied ICSI embryos (PGS)

* Arm 2: none biopsied ICSI embryos

* Arm 3: natural pregnancy embryos

Recruitment & Eligibility

Status
RECRUITING
Sex
Female
Target Recruitment
420
Inclusion Criteria
  1. Singleton pregnant women
  2. Pregnancy confirmation by ultrasound at week 6 of gestation either by normal pregnancy or ICSI (Biopsied and Non-Biopsied)
  3. Between the age of 20 and 40 years.
  4. Non-smokers.
  5. BMI must be between 19 and 40
Exclusion Criteria
  1. Those with anti-phospholipid syndrome confirmed by serological tests
  2. Those with any hematological and immunological disorders
  3. Women with uterine abnormalities have not been corrected.
  4. Women with systemic diseases that cannot be controlled or managed

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Arm 1Biopsied ICSI embryos (PGS)Biopsied ICSI embryos
Primary Outcome Measures
NameTimeMethod
Third primary endpointAt 3rd trimester of pregnancy

- Third-trimester U/S measurements of fetal growth : U/S measurements of fetal growth using growth curves and measurement of FPI (fetal ponderal index) and fetal weight percentile

First primary endpointAt pregnancy

- Early ultrasound measurements "yolk sac (YS), mean sac diameter (MSD), crown rump length (CRL)", different maternal serum prenatal screening markers for growth and aneuploidy between the three arms\* of the study

Second primary endpointAt 2nd trimester of pregnancy

- Second trimetric U/S measurements of fetal growth : U/S measurements of fetal growth using growth curves and measurement of FPI (fetal ponderal index) and fetal weight percentile

Secondary Outcome Measures
NameTimeMethod
First secondary endpointAt Labor

Miscarriage

Third secondary endpointAt Labor

Preterm labor

Second secondary endpointAt Labor

Live-birth weight

Trial Locations

Locations (1)

wael Elbanna Clinic

🇪🇬

Cairo, Egypt

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