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The INDORSE Study: Inhibition of Dipeptidyl Peptidase IV: Outcomes on Renal Sodium Excretion

Phase 4
Completed
Conditions
Type 2 Diabetes
Interventions
Drug: Sitaglitpin
Other: Placebo
Registration Number
NCT02406443
Lead Sponsor
University Health Network, Toronto
Brief Summary

Background: Dedicated renal hemodynamic and renal function studies are lacking for DPP-4 inhibitors in patients with Type 2 diabetes; accordingly little is known regarding the mechanisms mediating the renal effects of DPP-4 inhibitors in humans.

Objectives: To evaluate the effect of DPP-4 inhibition acutely (single dose) and following short-term therapy (28 days) on renal sodium handling and renal hemodynamics and function in patients with type 2 diabetes and systolic hypertension.

Design: double-blind, randomized, placebo-controlled trial, Phase IV.

Patient population: 32 patients with Type 2 diabetes, HbA1c (6.5%-9%), with systolic blood pressure ranging from 120-160 mmHg.

Intervention: subjects will be randomized (1:1) to either sitagliptin (100 mg daily) or to placebo (1 tablet daily) for 28 days.

Endpoints: Fractional excretion of sodium, renal function, and renal hemodynamics.

Detailed Description

Background: DPP-4 inhibition improves glycemic control, modestly reduces blood pressure and may also reduce albuminuria in patients with Type 2 diabetes; effects which occur without significantly modifying heart rate or body weight. While preclinical studies have demonstrated that DPP-4 inhibition acutely increases urinary sodium excretion in addition to other favorable renal effects (anti-inflammatory, anti-proteinuric), few studies have examined the renal effects of DPP-4 inhibition either acutely or following short-term therapy in humans with type 2 diabetes. Considering the world-wide prevalence of Type 2 diabetes and the increasing use of DPP-4 inhibitors amongst patients, it is important to ascertain potential non-glycemic effects of DPP-4 inhibitors including those within the kidney.

Study Objectives: To determine effect(s) of DPP-4 inhibition on tubular sodium handling, renal hemodynamics, and renal function.

Study Design: double-blind, randomized, placebo-controlled trial, Phase IV.

Study Patients: 32 patients with Type 2 Diabetes and Systolic Hypertension (SBP 120-160 mmHg).

Endpoints: Fractional excretion of sodium, renal function (measured GFR), renal hemodynamics (effective renal plasma flow, filtration fraction, renal blood flow, renal vascular resistance), systemic hemodynamics (non-invasive cardiac monitoring), plasma neurohormones, urinary vasoactive mediators, markers of free radical stress.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
36
Inclusion Criteria
  • Individuals of 18-70 years of age,
  • with Type 2 Diabetes,
  • with an HbA1c (6.5%-9%),
  • and with a systolic blood pressure (120-160 mmHg).
Exclusion Criteria
  • Individuals with:

    1. Type 1 Diabetes,
    2. eGFR <50mL/min/1.73m,
    3. pregnancy or breast feeding,
    4. significant cardiac, pulmonary or liver disease,
    5. prior history of pancreatitis, medullary thyroid cancer, multiple endocrine neoplasia syndromes,
    6. SBP >161 mmHg, 7) DBP >100 mmHg,
    7. alcohol or substance abuse,
    8. states of secondary hypertension.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Experimental armSitaglitpinsitagliptin (DPP-4 inhibitor) oral tablet (100 mg); Januvia; administered once daily for 28 days
Placebo armPlaceboplacebo (no medicinal ingredients) oral tablet (100 mg); administered once daily for 28 days
Primary Outcome Measures
NameTimeMethod
Percent Change in Fractional Excretion of Sodium (FENA)3 Hrs post-administration after 1 month and after 1 dose

FENA at 3Hrs post-study drug administration after 1 month compared to FENA at 3Hrs post-study drug administration after 1 dose expressed as percent change, sitagliptin vs. placebo

Secondary Outcome Measures
NameTimeMethod
Change in Glomerular Filtration Rate (GFR)3 Hrs post-administration after 1 month and after 1 dose

Measured GFR (Inulin Clearance) at 3Hrs post study-drug after 1 month compared to Measured GFR at 3Hrs post-study drug after 1 dose, sitagliptin vs. placebo

Change in Fractional Excretion of Lithium (FELi)3 Hrs post-administration after 1 month and after 1 dose

FELi at 3 Hr post-study drug administration after 1 month compared to FELI at 3hrs post-study drug administration after 1 dose, sitagliptin vs. placebo

Change From Baseline in SDF-1alpha^1-67 (Intact) Measured by Immunoaffinity and Tandem Mass Spectrometry3 Hr vs. baseline after 1 dose

Plasma concentration of SDF-1alpha\^1-67 (intact) measured by quantitative mass spectrometry methods after antibody-based affinity enrichment, sitagliptin vs. placebo

Change From Baseline in SDF-1alpha^3-67 (Truncated) Measured by Tandem Mass Spectrometry With Antibody-based Affinity Enrichment3Hrs vs baseline after 1 dose

Plasma concentration of SDF-1alpha\^3-67 (intact) measured by quantitative mass spectrometry methods after antibody-based affinity enrichment, sitagliptin vs. placebo

Change in Systolic Blood Pressure (SBP), Non-invasive Cardiac Output Monitoring3 Hrs post-administration after 1 month and after 1 dose

SBP by Non-Invasive cardiac output monitoring at 3Hrs post- study drug administration after 1 month compared to SBP by Non-invasive cardiac output monitoring at 3Hrs after 1 dose, sitagliptin vs placebo

Change in Effective Renal Plasma Flow (ERPF)3 Hrs post-administration after 1 month and after 1 dose

ERPF (para-aminohippurate clearance) 3Hrs post-study drug administration after 1 month compared to ERPF at 3Hhrs post-study drug administration after 1 dose, sitagliptin vs placebo

Trial Locations

Locations (1)

University Health Network - Division of Nephrology

🇨🇦

Toronto, Ontario, Canada

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