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Pilot Phase I/II Study of Amino Acid Leucine in Treatment of Patients With Transfusion-Dependent Diamond Blackfan Anemia

Phase 1
Completed
Conditions
Diamond Blackfan Anemia
Congenital Hypoplastic Anemia
Pure Red Cell Aplasia
Blackfan Diamond Syndrome
DBA
Interventions
Registration Number
NCT01362595
Lead Sponsor
Northwell Health
Brief Summary

This study will determine the safety and possibility of giving the amino acid, leucine, in patients with Diamond Blackfan anemia(DBA)who are on dependent on red blood cell transfusions.

The leucine is expected to produce a response in patients with DBA to the point where red blood cell production is increased. Red cell transfusions can then be less frequent or possibly discontinued.

The investigators will study the side effects, if any, of giving leucine to DBA patients. Leucine levels of leucine will be obtained at baseline and during the study.

The drug leucine will be provided in capsule form and taken 3 times a day for a total of 9 months.

Detailed Description

Leucine will be provided to participants in the form of a capsule and will be taken three times daily.

Blood hemoglobin levels will be monitored every 3-4 weeks for 9 months.

The entire study will last 12-15 months in length.

Subjects must be two years of age or older and on transfusion for more than six months prior to enrollment.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
55
Inclusion Criteria
  • diagnosed with Diamond Blackfan anemia as published in British Journal of Hematology
  • transfusion dependent
  • age 2 years and older
  • adequate renal function
  • adequate liver function
  • negative B-HCG if patient is a menstruating female and documentation of adequate contraception
  • signed informed consent
Exclusion Criteria
  • Known hypersensitivity to branched chain amino acids
  • Diagnosis of an inborn error of amino acid metabolism disorder
  • Prior hematopoietic stem cell transplantation
  • Pregnancy, or plans to become pregnant during duration of trial

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
LeucineleucineNo alternative treatment arm
Primary Outcome Measures
NameTimeMethod
Response to Leucine in Transfusion Dependent Patients With Diamond Blackfan Anemia9 Months

The primary outcome is the type of response observed at 9 months. Response to treatment can be one of the following:

1. Complete response (CR): Hb \> 9 gm/dL and transfusion-independence as defined in DBA

2. Partial response (PR): Hb \< 9 gm/dL and increased reticulocyte count to greater than baseline.

3. No response (NR): no change in transfusion requirements and no significant change reticulocyte count from baseline

4. Progression: worsening of disease as defined by the need for more frequent transfusions

Secondary Outcome Measures
NameTimeMethod
Severe Adverse Events Attributable to Leucine9 months

Adverse events occurring while participants were on Leucine

Trial Locations

Locations (11)

Boston Children's Hospital

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Boston, Massachusetts, United States

University of Missouri-Columbia Women's and Children's Hospital

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Columbia, Missouri, United States

UT Southwestern Medical Center

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Dallas, Texas, United States

Stanford University Medical Center

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Palo Alto, California, United States

Riley Hospital for Children

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Indianapolis, Indiana, United States

Children's Specialty Center of Nevada

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Las Vegas, Nevada, United States

Cohen Children's Medical Center of New York

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New Hyde Park, New York, United States

Phoenix Children's Hospital

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Phoenix, Arizona, United States

Children's Hospital of Philadelphia

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Philadelphia, Pennsylvania, United States

University of Louisville

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Louisville, Kentucky, United States

University of Michigan C.S. Mott Children's Hospital

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Ann Arbor, Michigan, United States

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