Obesity and Oxidative Stress in Patients With Different Periodontal Status: A Cross-sectional Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 93
- 主要终点
- Protein Carbonyl levels in gingival crevicular fluid as measured an oxidative stress marker in proteins
研究概览
简要总结
The aim of the study is to evaluate the effect of obesity on periodontally healthy or diseased tissues.Normal weight and obese subjects were analyzed in 6 groups. Clinical periodontal status was determined through plaque index, gingival index, bleeding on probing, pocket probing depth, clinical attacment level and radiographs.
Oxidative stress is known to contribute to various diseases by affecting cellular functions through the oxidation of proteins, lipids and DNA. Oxidative stress can be assessed by measuring the products of oxidative damage found in proteins, lipids and DNA or reductions in total antioxidant capacity Malondialdehyde, protein carbonyl and total antioxidant capacity levels are biomarkers of oxidative stress in gingival crevicular fluid.
详细描述
This cross-sectional study was conducted with 93 subjects (45 normal-weight, and 48 class I obese) recruited from the Periodontology Department at the Ondokuz Mayıs University Faculty of Dentistry; Endocrinology and Metabolic Diseases Department at the Ondokuz Mayıs University Faculty of Medicine in Turkey, between September 2012 and March 2014.
The study protocol was approved by the Local Ethics Committee, and written informed consent was obtained from all study participants in accordance with the Helsinki Declaration (revised in 2000).
Oxidative stress occurs when the balance between reactive oxygen species and antioxidants become unbalanced. OS can be determined by evaluation of oxidative damage products in proteins, lipids and DNA or the reduction of total antioxidant capacity.
Malondialdehyde level is the biomarker of oxidative stress in lipids. Protein carbonyl level is the biomarker of oxidative stress in proteins.Total antioxidant capacity level is another biomarker of oxidative stress. Malondialdehyde, protein carbonyl and total antioxidant levels in crevicular fluid were examined by ELISA method.
Obesity was diagnosed according to World Health Organization criteria using body mass index. Body mass index is defined as a person's weight, in kilograms (kg), divided by the square of height in meters (m), and it is classified as follows:
研究设计
- 研究类型
- Observational
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 35 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •(i) ≥ 18 years of age and having ≥ 16 teeth; (ii) no periodontal therapy in the 6 months prior to data collection; (iii) no systemic problems or chemotherapy within the 6 weeks prior to data collection; (iv) no previous history of smoking.
排除标准
- •(i) medical history of cancer, rheumatoid arthritis, diabetes mellitus, or cardiovascular disease; (ii) compromised immune system; (iii) pregnancy, menopause, or lactation; (iv) ongoing drug therapy that might affect the clinical characteristics of periodontitis; (v) use of systemic antimicrobials during the 6 weeks prior to data collection; (vi) dental treatment during the 6 months prior to data collection.
结局指标
主要结局
Protein Carbonyl levels in gingival crevicular fluid as measured an oxidative stress marker in proteins
时间窗: between 8-10 am on the day following periodontal status assessment.
Protein carbonyl is another biomarker of oxidative stress
Malondialdehyde levels in gingival crevicular fluid as measured an oxidative stress marker in lipids
时间窗: between 8-10 am on the day following periodontal status assessment.
Malondialdehyde is the most specific and most used molecule for measurement of biological lipid oxidation.
Reduction of total antioxidant capacity levels in gingival crevicular fluid as measured an oxidative stress marker
时间窗: between 8-10 am on the day following periodontal status assessment.
Total antioxidant capacity provides an overview of the biological interaction between an individuals' antioxidant status and how well these antioxidants are able to protect host cells during periods of oxidative stress. Due to the potential synergistic effects of different antioxidant molecules, the measurement of total antioxidant capacity can provide a more accurate assessment of antioxidant status than the separate measurement of individual molecules.
次要结局
- Plaque index(the day before gingival crevicular fluid samples were collected)
- Pocket probing depth(the day before gingival crevicular fluid samples were collected)
- Gingival index(the day before gingival crevicular fluid samples were collected)
- Bleeding on probing(the day before gingival crevicular fluid samples were collected)
- Clinical attachment level(the day before gingival crevicular fluid samples were collected)
研究者
Vadim Ekrem Atabay
phd
Ondokuz Mayıs University