The effects of different fat structures on postprandial responses in healthy subjects.

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 40

Inclusion Criteria

- Apparently healthy man or woman
- Age 40-70y at the time of recruitment
- BMI of 22-27 kg/m2
- Having a Hb value above 8.4 (men) or 7.4 (women) mmol/L (will be checked at
the screening visit)
- Having veins suitable for blood sampling via a catheter cannula (judged by
study nurse/ medical doctor)
- Having a general practitioner
- Signed informed consent

Exclusion Criteria

- Any chronic metabolic, gastrointestinal, inflammatory or chronic disease
(such as diabetes, anaemia, hepatitis, cardiovascular disease)
- History of gastro-intestinal surgery or having (serious) gastro-intestinal
complaints
- Renal or hepatic malfunctioning (pre-diagnosis or determined based on ALAT,
ASAT and creatinine values)
- Use of medication that may influence the study results, such as laxatives,
stomach protectors and drugs that can affect intestinal motility.
- Donated or intend to donate blood from 2 months before the study until the
end of the study
- Reported slimming, medically prescribed or vegan diet
- Unstable body weight (weight gain or loss >5 kg in the past three months)
- Current smokers
- Alcohol on average: more than 2 consumptions/day or more than 14
consumptions/week
- Pregnant, lactating or wishing to become pregnant in the period of the study
(self-reported)
- Use of drugs (soft and/or hard drugs)
- Food allergies for products that we use in the study
- Participation in another clinical trial at the same time, or in the month
preceding the start of this study
- Inability to understand study information and/or communicate with staff
- Members of the research team
- Working, or doing an internship or thesis at the division *Human Nutrition
and Health*, Wageningen University

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1) whole genome gene expression changes in isolated monocytes from the<br /><br>circulation, 2) postprandial changes in cytokine concentrations in the<br /><br>circulation, and 3) functional responses and sensitivity of the isolated<br /><br>monocytes from the circulation.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>1) postprandial triglyceride concentration and other cardio-metabolic markers<br /><br>in the circulation, 2) postprandial feelings of hunger and satiety, and 3)<br /><br>changes in cytokine concentrations in the circulation measured in dried blood<br /><br>spots. </p><br>