Collagen digestion and amino acid absorption kinetics and the effect on muscle & skin connective tissue protein synthesis in vivo in humans

• Conditions: Digestie & absorptie, spier en huid; Muscle connective tissue protein synthesis; muscle connective tissue buildup

• Registration Number: NL-OMON53526
• Lead Sponsor: niversiteit Maastricht

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 29 April 2024

Recruitment & Eligibility

• Status: Pending
• Sex: Not specified
• Target Recruitment: 14

Inclusion Criteria

- Aged between 18-35 years
- Healthy, recreationally active (participating in recreational sports
activities >= 1 and <= 6 h per week, with a maximum of 2 h resistance-type
exercise)
- 18.5 <= BMI <= 30 kg/m2
- No physical limitations (i.e. able to perform all activities associated with
daily living in an independent manner).

Exclusion Criteria

- Smoking
- Musculoskeletal disorders
- Metabolic disorders
- Use of any medications known to affect protein metabolism (i.e.
corticosteroids, non-steroidal anti-inflammatories, or prescribed acne
medications).
- Chronic use of gastric acid suppressing medication or anti-coagulants
- Unstable weight over the last three months
- Diagnosed GI tract disorders or diseases
- Blood donation in the past 2 months

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The primary endpoint will be the muscle connective tissue protein synthesis<br /><br>over the period before and after intake of collagen protein peptides, as<br /><br>determined using stable isotope tracer methodology. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>To quantify the digestion and absorption kinetics of collagen peptides and the<br /><br>subsequent impact on skin connective tissue protein synthesis rates in vivo in<br /><br>humans. Also, incorporation of collagen-derived amino acids in muscle &amp;<br /><br>skin tissue will be determined. Other secondary outcomes include whole-body<br /><br>protein kinetics (total rate of appearance, exogenous rate of appearance,<br /><br>endogenous rate of appearance, rate of disappearance), whole-body protein<br /><br>metabolism (synthesis, breakdown, oxidation, net balance), overall plasma amino<br /><br>acid responses, postprandial hydroxyproline concentration over time (iAUC) and<br /><br>plasma insulin responses.</p><br>