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Alemtuzumab and Rituximab in Treating Patients With High-Risk, Early-Stage Chronic Lymphocytic Leukemia

Phase 2
Completed
Conditions
Leukemia
Interventions
Registration Number
NCT00436904
Lead Sponsor
Mayo Clinic
Brief Summary

RATIONALE: Monoclonal antibodies, such as alemtuzumab and rituximab, can block cancer growth in different ways. Some block the ability of cancer cells to grow and spread. Others find cancer cells and help kill them or carry cancer-killing substances to them. Giving alemtuzumab together with rituximab may kill more cancer cells.

PURPOSE: This phase II trial is studying the side effects and how well giving alemtuzumab together with rituximab works in treating patients with high-risk, early-stage chronic lymphocytic leukemia.

Detailed Description

OBJECTIVES:

Primary

* Determine the rate of complete and overall response to alemtuzumab and rituximab in patients with high-risk, early-stage chronic lymphocytic leukemia.

* Determine the toxicity of this regimen in these patients. Secondary

* Determine the overall survival and time to progression of patients treated with this regimen.

* Determine time to response and duration of response in patients treated with this regimen.

* Correlate prognostic markers 11q-, 17p-, unmutated VH gene, and CD38+ with clinical outcome.

* Determine response to this regimen using an expanded definition of response that includes minimal residual disease detected by sensitive flow cytometry in patients in complete clinical remission and single rearranged IgVH gene detected by polymerase chain reaction in patients with no monoclonal population on flow cytometry.

* Correlate in vitro response with clinical outcome in patients treated with this regimen.

* Determine if alemtuzumab and rituximab are synergistic in vitro.

* Determine the mechanism of action of this regimen in vitro.

* Determine the effect of this regimen on immune function.

* Monitor T-lymphocyte, natural killer cell, and monocyte number during and after treatment in these patients.

* Serially evaluate T-lymphocyte immunophenotype and function in patients treated with this regimen.

* Monitor recovery of humoral immunity by serial serum protein electrophoresis, immunofixation electrophoresis, and immunoglobulin quantification.

OUTLINE:

* Dose-escalation (week 1): Patients receive rituximab IV on day 1 and escalating doses of alemtuzumab subcutaneously (SC) on days 3-5 in week 1.

* Treatment (weeks 2-5): Patients receive alemtuzumab SC on days 1-3 (at the highest dose administered during week 1) and rituximab IV on day 3 in weeks 2-5 in the absence of disease progression or unacceptable toxicity.

Patients undergo blood collection at baseline and periodically during study treatment for pharmacokinetic and prognostic biomarker (11q-, 17p-, unmutated IgVH, and CD38 expression by flow cytometry and fluorescent in-situ hybridization) studies. Immune function (CDR3 T-cell receptor by reverse transcriptase-polymerase chain reaction) and in vitro and in vivo response are also examined.

After completion of study therapy, patients are followed periodically for 5 years.

PROJECTED ACCRUAL: A total of 33 patients will be accrued for this study.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
30
Inclusion Criteria

Not provided

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Exclusion Criteria

Not provided

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Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Alemtuzumab + RituximabRituximabAlemtuzumab 30mg Monday, Wednesday, and Friday x 5 weeks, Rituximab 375/mg/m2 IV weekly (Wednesday) x 4 weeks (weeks 2-5)
Alemtuzumab + RituximabAlemtuzumabAlemtuzumab 30mg Monday, Wednesday, and Friday x 5 weeks, Rituximab 375/mg/m2 IV weekly (Wednesday) x 4 weeks (weeks 2-5)
Primary Outcome Measures
NameTimeMethod
Number of Participants With Treatment Related Adverse EventsWeekly for first 6 weeks, then monthly for 6 months, then at 9 and 12 months post registration

Adverse events (AE) that are classified as either possibly, probably, or definitely related to study treatment according to the National Cancer Institute Common Toxicity Criteria for Adverse Events (NCI CTCAE version 3.0). The maximum grade for each type of AE will be recorded for each patient. Grade refers to the severity of the AE.\>

\> Grade 1: Mild AE, Grade 2: Moderate AE, Grade 3: Severe AE, Grade 4: Life-threatening or disabling AE, Grade 5: Death related AE

Confirmed Response, Defined as Objective Complete Remission or Partial Remission for a Duration of at Least 2 MonthsUp to 6 months

Confirmed response is defined as a \> 50% decrease in clinical symptoms from baseline and recovery from blood counts.

Secondary Outcome Measures
NameTimeMethod
SurvivalDeath or last follow-up (up to 5 years)

Survival is calculated from the date of registration to the date of death due to any cause. In patients who are still alive, survival will be censored at the last date when the patient was known to be alive.

Time to Disease ProgressionTime from registration to progression (up to 5 years)

Calculated from date of registration to date of disease progression. In patients that have not progressed, time to disease progression will be censored at the patient's last evaluation date.

Time to ResponseRegistration to first response (up to 5 years)

Calculated from the date of registration until the first date at which the patient's objective status was classified as a response. In patients who do not achieve a response, time to response will be censored at the patient's last evaluation date. Response is defined the same way as in the response primary outcome measure.

Duration of ResponseUp to 5 years

Duration of response is calculated from the date of documented response until the date of progression in the subset of patients who respond to treatment. In patients who have not yet progressed, duration of response will be censored at the patient's last evaluation date.

Trial Locations

Locations (1)

Mayo Clinic

🇺🇸

Rochester, Minnesota, United States

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