Absorption, Metabolism and Excretion of Artichoke Polyphenols in Healthy Individuals

Not Applicable

Completed

• Conditions: Healthy Men and Women
• Interventions: Dietary Supplement: Artichokes

• Registration Number: NCT04095949
• Lead Sponsor: King's College London

Brief Summary

A number of studies have reported the presence of polyphenolic compounds in artichokes, being the most abundant the caffeoylquinic acids (CQAs), which are esters between quinic acid and the hydroxycinnamate caffeic acid. The fate of artichoke polyphenols in humans after ingestion is currently unknown. The acute study will provide novel information on the bioavailability and metabolism of artichoke polyphenols, which will give important insights to understand the potential health benefits of artichoke consumption.

Health men and women participants (n=8) aged between 18 to 40 years old will be recruited. Participants will consume a normal ration of artichokes once. The participants will provide the investigators with urine and blood samples for polyphenol analysis at baseline and at different time points until 24 hours.

Detailed Description

Not available

Study Timeline

• Study Start: 2019-07-17
• Study First Submitted: 2019-07-24
• Primary Completion: 2019-07-30
• Study Completion: 2019-07-30
• Study First Submitted QC: 2019-09-18
• Study First Posted: 2019-09-19
• Status Verified: 2019-10
• Last Update Submitted: 2019-10-10
• Last Update Posted: 2019-10-14

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 8

Inclusion Criteria

1. Healthy men and women aged 18-40 years old
2. Subjects are willing to maintain their normal eating/drinking habits and exercise habits to avoid changes in body weight over the duration of the study
3. Are able to understand the nature of the study
4. Able to give signed written informed consent
5. Signed informed consent form

Exclusion Criteria

1. Metabolic disease
2. Chronic disease or medications
3. BMI not between 18.5 and 30
4. Allergies to artichokes or other significant food allergy.
5. Subjects under medication or on vitamin/dietary supplements.
6. Subjects who reported participant in another study within one month before the study start
7. Pregnant woman or planning to become pregnant in the next month
8. Any reason or condition that in the judgment of the clinical investigator(s) may put the subject at unacceptable risk or that may preclude the subject from understanding or complying with the study's requirements.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Artichokes	Artichokes	1 day of artichokes intake

Primary Outcome Measures

Name	Time	Method
Change in qualitative identification of polyphenols and their metabolites of artichokes in urine between baseline and 24h post consumption	Baseline, 0-2 hours, 2-4 hours, 4-8 hours and 8-24 hours	Change in polyphenols and their metabolites in urine between baseline and 24h post consumption will be measured by identifying qualitatively metabolites from artichokes at different time points using chromatography coupled with mass spectrometry analytical techniques (UPLC-MS).
Change in qualitative identification of polyphenols and their metabolites of artichokes in plasma between baseline and 24h post consumption	Baseline, 0.5 hours, 1 hour, 2 hours, 4 hours, 6 hours and 24 hours	Change in polyphenols and their metabolites in plasma between baseline and 24h post consumption will be measured by identifying qualitatively metabolites from artichokes at different time points using chromatography coupled with mass spectrometry analytical techniques (UPLC-MS).
Change in the plasmatic concentration of polyphenols (nanomol/L) and their metabolites of artichokes between baseline and 24h post consumption	Baseline, 0.5 hours, 1 hour, 2 hours, 4 hours, 6 hours and 24 hours	Bioavailability and metabolism from artichoke polyphenols will be studied by evaluating changes in artichoke polyphenols concentration (nanomol/L) in plasma samples at different time points using chromatography coupled with mass spectrometry analytical techniques (UPLC-MS).
Change in the concentration of polyphenols in urine (nanomol/L) and their metabolites of artichokes between baseline and 24h post consumption	Baseline, 0-2 hours, 2-4 hours, 4-8 hours and 8-24 hours	Metabolism and excretion from artichoke polyphenols will be studied by evaluating changes in artichoke polyphenols concentration (nanomol/L) in urine samples at different time points using chromatography coupled with mass spectrometry analytical techniques (UPLC-MS).

Trial Locations

Locations (1)

King's College London, Department of Nutritional Sciences; 🇬🇧 London, United Kingdom