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Anthocyanin & Phenolic Acids Bioavailability & Antioxidant Capacity After Consumption of Purple Wheat Products in Adults

Not Applicable
Completed
Conditions
Healthy
Interventions
Other: Purple Wheat Crackers
Other: Purple Wheat Granola Bars
Registration Number
NCT02730910
Lead Sponsor
University of Guelph
Brief Summary

This study will determine the bioavailability of anthocyanins and phenolic compounds from two purple wheat products, i.e. bran-enriched wholegrain purple wheat crackers and bran-enriched wholegrain purple wheat granola bars. Crackers are an example of a baked/ processed product. The purple wheat granola bars are an example of a food product that requires much less intensive preparation and cooking, compared to the crackers. A comparison of bioavailability between these two products should provide insights about how different processes and food matrix influence anthocyanin bioavailability and metabolism.

Detailed Description

This study will determine the bioavailability of anthocyanins and phenolic compounds from two purple wheat products, i.e. bran-enriched wholegrain purple wheat crackers and bran-enriched wholegrain purple wheat granola bars. Crackers are an example of a baked/ processed product. The purple wheat granola bars are an example of a food product that requires much less intensive preparation and cooking, compared to the crackers. A comparison of bioavailability between these two products should provide insights about how different processes and food matrix influence anthocyanin bioavailability and metabolism. The study is a crossover investigation with 8 men and 8 women who will adhere to a low anthocyanin and low phenolic diet for the duration and complete 2 postprandial study visits where, in random order, they will consume either the crackers or granola bars. Blood and urine will be collected at baseline and postprandially for the analysis of anthocyanins and their metabolites and phenolic acids and determination of antioxidant capacity.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
16
Inclusion Criteria
  • BMI of 18 - 27 kg/m2
  • Generally healthy as determined by screening questionnaire
  • Non-smoking
  • Non to moderate alcohol drinkers (Typical consumption less than 5 drinks per sitting and no more than 14 per week)
Exclusion Criteria
  • Pregnant or breast-feeding females
  • History of any major disease or medical condition, including any disorder of the gastrointestinal system or food intolerances
  • Taking any prescription medications (including hormonal contraceptives) or over the counter medications prescribed by a medical professional
  • Taking any natural health products or dietary supplements, other than a multivitamin low in anthocyanins and phenolic acids
  • Oral antibiotic use in the past 3 months
  • Any food allergies, and any anaphylactic or life-threatening allergies - food or otherwise
  • Smokers or use of recreational drugs (marijuana, magic mushrooms, etc)
  • Elite or training athletes
  • Following a vegan or vegetarian diet

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Purple Wheat CrackersPurple Wheat CrackersBran-enriched purple wheat wholegrain crackers served in 120 g portion.
Purple Wheat Granola BarsPurple Wheat Granola BarsBran-enriched purple wheat wholegrain granola bars served in 160 g portion.
Primary Outcome Measures
NameTimeMethod
Plasma concentrations of anthocyanin metabolites0-8 hours (0, 0.5, 1, 1.5, 2, 2.5, 3, 4, 6, 8)
Secondary Outcome Measures
NameTimeMethod
Plasma concentrations of phenolic acids0-8 hours (0, 0.5, 1, 1.5, 2, 2.5, 3, 4, 6, 8)
Plasma antioxidant capacity0-8 hours (0, 2, 4, 6, 8)
Urinary concentrations of phenolic acids0-8 hours (0, 0.5, 1, 1.5, 2, 2.5, 3, 4, 6, 8)
Urinary concentrations of anthocyanin metabolites0-8 hours (0, 0.5, 1, 1.5, 2, 2.5, 3, 4, 6, 8)

Trial Locations

Locations (1)

Human Nutraceutical Research Unit, University of Guelph

🇨🇦

Guelph, Ontario, Canada

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