Single-cell RNA Sequencing Resolves the Regulatory Role of HBV on the Hepatocellular Carcinoma Immune Microenvironment
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- single cell RNA sequencing
研究概览
简要总结
In summary, with the help of single-cell sequencing technology, this study aims to focus on elucidating the influence of HBV-induced hepatocellular carcinoma cell metabolic changes on microenvironment remodeling. With the help of hepatocellular carcinoma microenvironment changes, this study provide a more accurate diagnosis and treatment method for HBV-induced hepatocellular carcinoma.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients with primary liver cancer (hepatocellular carcinoma (HCC)), with evidence of histological or cytological diagnosis, or with HCC meeting conventional clinical diagnostic criteria.
- •Both sexes, aged 18-80 years old
- •The results of HBVDNA test were in line with the inclusion criteria
- •The patient had at least one measurable lesion according to the Response Evaluation Criteria in Solid Tumors, version 1.1 (RECIST V1.1)
- •Agreed to comply with the study protocol for treatment and follow-up, agreed to provide clinicopathological and follow-up data required by the study, and agreed to use the study data for subsequent research and product development
排除标准
- •Other malignant tumors;
- •Patients with severe organic diseases do not meet the requirements of infectious liver cancer in this study;
- •Suffering from mental illness cannot guarantee the compliance of this study;
- •Previous recipients of any cell or organ transplantation;
- •Received local regional liver therapy (including various ablations, percutaneous ethanol or acetic acid injections, high-intensity focused ultrasound, transarterial embolization, chemotherapy, or chemoembolization plus embolization) within 14 days prior to study treatment initiation.
结局指标
主要结局
single cell RNA sequencing
时间窗: within 4 hours after the sample was submitted for examination
10x genomics chromium 3' sequencing
Biochemical index detection
时间窗: within 4 hours after the sample was submitted for examination
HbsAg (IU/mL) \\HbsAb (mIU/mL)\\HbeAg\\HbeAb\\HbcAb
HBV DNA copy number
时间窗: within 24 hours after the sample was submitted for examination
HBV DNA (IU/mL)
次要结局
未报告次要终点
研究者
Fubing Wang
Dr.Prof.
Zhongnan Hospital