The Effect of Narrow Band UVB on E-cadherin and Integrin Alpha v Beta 1 in Non-segmental Vitiligo: A Novel Proposed Mechanism
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 15
- 试验地点
- 2
- 主要终点
- Melanocyte marker (Melan A) for staining of melanocytes
研究概览
简要总结
The purpose of this clinical trial is to study the effect of Narrow Band UVB on E-cadherin and integrin alpha v beta 1 as representatives of adhesion molecules in non-segmental vitiligo in a trial to verify a novel proposed mechanism of Narrow Band UVB. Additionally, specific melanocyte marker (Melan A) will be done to settle the localization of adhesion molecules in relation to melanocytes.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Sequential
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •All clinically diagnosed cases of non-segmental vitiligo .
- •Patients with VIDA +1 or more (new/expanding lesions active in time period of 6-12 months ago) will be included in the trial. Activity of vitiligo will be evaluated through Vitiligo Disease Activity (VIDA) Score.
排除标准
- •Patients who have stable disease at the time of study i.e. no progression of existing disease or appearance of new lesions in the last six months.
- •Patients receiving treatment for vitiligo 3 months prior to the study.
- •Patients with contraindications for phototherapy.
- •Patients with current/history of any systemic and/or dermatological diseases affecting the immune system as autoimmune diseases and malignancy.
结局指标
主要结局
Melanocyte marker (Melan A) for staining of melanocytes
时间窗: 4 months
Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields.
E-cadherin expression as a cell adhesion molecule between melanocytes and keratinocytes
时间窗: 4 months
Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields.
Integrin alpha v beta 1 expression as a cell adhesion molecule between melanocytes and keratinocytes
时间窗: 4 months
Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields.
次要结局
- Vitiligo Area and severity index (VASI)(4 months)
研究者
Rana F Hilal, MD
Lecturer of dermatology
Kasr El Aini Hospital