Comparison of Human Embryonic Development Using Sequential and Single Media in IVF Monitored by Time-lapse Imaging.
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Infertility
- Sponsor
- Vitrolife
- Enrollment
- 128
- Locations
- 4
- Primary Endpoint
- Percentage of good quality blastocysts
- Status
- Completed
- Last Updated
- 5 years ago
Overview
Brief Summary
The purpose of this study is to compare human embryo in vitro development in sequential and single-step culture medium using time-lapse imaging.
Detailed Description
Objective: To study whether a culture medium that allows undisturbed culture supports human embryo development to the blastocyst stage equivalently to a well-established sequential media. Design: Randomized, double-blinded sibling trial. Setting: Independent in vitro fertilization (IVF) clinics. Patient(s): One hundred twenty-eight patients, with 1,356 zygotes randomized into two study arms. Intervention(s): Embryos from each patient were randomly allocated into two study arms to compare embryo development on a time-lapse system using a single-step medium or sequential media.
Investigators
Eligibility Criteria
Inclusion Criteria
- •6 or more normal embryos following egg retrieval and fertilization.
- •Female patients aged 21-40 years or 21-55 years who are using an egg donor.
- •Patients using fresh or frozen ejaculated sperm
Exclusion Criteria
- •Previous history of cancer
- •Day 3 embryo transfer
Outcomes
Primary Outcomes
Percentage of good quality blastocysts
Time Frame: 5 days of in vitro embryo culture
A cohort of human embryos will be divided into two different media. The ability of the media to support human embryo development will be determined using the standard assessment of the percentage of embryos that achieve a defined embryo grade.
Secondary Outcomes
- % GQE DAY 3(Measured 3 days after oocyte pick-up)
- Blast. rate(Measured 5 and 6 days after oocyte pick-up.)
- Blast. Utilization rate(Measured 5 and 6 days after oocyte pick-up)