n3 PUFA and Muscle-disuse Atrophy in Young Women

Not Applicable

Completed

• Conditions: Muscle Atrophy
• Interventions: Dietary Supplement: n3 PUFA-enriched fish oil; Dietary Supplement: Placebo

• Registration Number: NCT03059836
• Lead Sponsor: McMaster University

Brief Summary

This study will examine the influence of n3 PUFA supplementation on the rate of muscle atrophy in women undergoing 2 weeks of unilateral limb immobilization. Assessments in skeletal muscle strength and skeletal muscle volume will also me made before, after and in recovery from immobilization.

Detailed Description

Biological aging is associated with the loss of skeletal muscle mass and strength resulting in compromised metabolic function and mobility. Throughout life, individuals will also experience periods of reduced physical activity/muscle disuse that independently lower muscle mass and strength accelerating the aging process. The losses in muscle mass with aging and disuse are underpinned by feeding-induced declines in rates of muscle protein synthesis. Thus, strategies to enhance muscle protein synthesis could have clinical implications for those who wish to maintain metabolic health and function during times of muscle disuse.

Supplementation with n3 PUFA-enriched fish oil has been shown to potentiate rates of muscle protein synthesis in response to simulated feeding in both younger and older adults. Fish oil supplementation also has been efficacious in enhancing skeletal muscle strength during a period of resistance exercise training. However, no study has examined the impact of fish oil supplementation to enhance muscle protein synthesis and offset declines in muscle mass/strength during a period of immobilization.

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study First Submitted: 2017-02-15
• Study First Submitted QC: 2017-02-16
• Study First Posted: 2017-02-23
• Study Start: 2017-02-28
• Primary Completion: 2018-02-28
• Study Completion: 2018-02-28
• Status Verified: 2018-03
• Last Update Submitted: 2018-03-20
• Last Update Posted: 2018-03-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 20

Inclusion Criteria

• Female
• Aged 18-30 years

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Exclusion Criteria

• Take any analgesic or anti-inflammatory drugs(s), prescription or non-prescription, chronically will be excluded
• A history of neuromuscular problems or muscle and/or bone wasting diseases
• Any acute or chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other metabolic disorders-all ascertained through medical history screening questionnaires
• Use medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications)
• Fish allergy

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
n3 PUFA	n3 PUFA-enriched fish oil	Intervention: n3 PUFA (3000mg of Eicosapentaenoic acid per day and 1800mg of Docosahexaenoic acid per day)
Placebo	Placebo	Intervention: Organic Sunflower Oil 5000mg per day

Primary Outcome Measures

Name	Time	Method
Muscle Volume	Baseline (0 days), After (14 days) of unilateral limb immobilization and at 14 days post immobilization	Change in Muscle Volume Measured by Magnetic Resonance Imaging

Secondary Outcome Measures

Name	Time	Method
Skeletal Muscle Strength	Before (0 days), After (14 days) of unilateral limb immobilization and at 14 days Post immobilization	Change in Skeletal Muscle Strength
Muscle Lipid Composition	Baseline (-28 days), and at Before (0 days), After (14 days) of unilateral limb immobilization and at 14 days Post immobilization	Change in Muscle Lipid Composition
Integrated Rates of Muscle Protein Synthesis	Before (0 days), During (0-14 days inclusive), and After (14-28 days inclusive) of unilateral limb immobilization	Change in Muscle Protein Synthesis

Trial Locations

Locations (1)

Exercise Metabolism Research Laboratory, McMaster Univeristy; 🇨🇦 Hamilton, Ontario, Canada