Healthy Diets for a Healthy Weight: Exploring Physiological Mechanisms Related to Dietary Fibre and Non-Nutritive Sweeteners
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Appetitive Behavior
- Sponsor
- University of Aberdeen
- Enrollment
- 20
- Locations
- 1
- Primary Endpoint
- Change in gut metabolites (from faecal samples) in response to dietary fibre and non-nutritive sweetener
- Status
- Completed
- Last Updated
- last year
Overview
Brief Summary
The investigators present a weight loss diet intervention study, to be conducted as a within-subject design, with all food and beverages provided, to assess interaction of non-nutritive sweetener (sucralose) with a high-fibre weight loss diet, on markers of gut health in humans. This study will allow assessment of the effects of a non-nutritive sweetener (sucralose) with a high-fibre (soluble fibre, fructo-oligosaccharides, FOS) diet on metabolic health and activity and composition of gut microbiota, by a controlled human diet intervention study. The investigators propose to recruit participants living with obesity, with a poor diet quality (moderate habitual fibre intake) to additionally address diet inequalities in the research approach, and this will also allow examine the time-course of adaptation of the gut microbiome (measured in faecal samples). The investigators will also assess changes in free-living glycaemic control with addition of dietary fibre and bio-markers of health.
Investigators
Eligibility Criteria
Inclusion Criteria
- •healthy but overweight/obese (BMI 28-40kg/m2) males and females (postmenopausal, using the oral contraceptive pill or some form of hormonal contraceptive)
- •moderate habitual fibre intake (18-23g/day)
Exclusion Criteria
- •Medication exclusion criteria:
- •antibiotic use (within the past 3 months due to impact on gut microbiota)
- •anti-depressants (current)
- •smoking or vaping
- •weight loss medication
- •Medical exclusion criteria:
- •Females who are planning to be pregnant, are pregnant or are breastfeeding
- •Anyone with food allergies, self-reported food sensitivity or intolerance
- •Anyone with coeliac disease or gluten intolerance
- •Anyone taking medication which may affect their appetite
Outcomes
Primary Outcomes
Change in gut metabolites (from faecal samples) in response to dietary fibre and non-nutritive sweetener
Time Frame: At baseline and end of each arm (study days 1, 15, 29 and 43)
Measured by changes in faecal short chain fatty acids concentrations
Change in gut microbiome composition (from faecal samples) in response to dietary fibre and non-nutritive sweetener
Time Frame: At baseline and end of each arm (study days 1, 15, 29 and 43)
Measured by changes in bacterial 16S ribosomal ribonucleic acid gene sequencing; 16S rRNA
Change in gut metabolite production (from faecal samples) in response to dietary fibre and non-nutritive sweetener
Time Frame: At baseline and end of each arm (study days 1, 15, 29 and 43)
Measured by changes in calprotectin concentrations
Secondary Outcomes
- Change in glycaemic control in response to dietary fibre and non-nutritive sweetener(At baseline and end of each arm (study days 1, 15, 29 and 43))
- Change in free-living glycaemic control in response to dietary fibre and non-nutritive sweetener(every day of study diets (42days))