Development of a Method for Assessing the Functional Activity of Platelets for the Differential Diagnosis of Thrombocytopathies Using Flow Cytometry
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Immune Thrombocytopenia
- Sponsor
- Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus
- Enrollment
- 200
- Primary Endpoint
- Expression of CD41 by platelets
- Status
- Not yet recruiting
- Last Updated
- 3 years ago
Overview
Brief Summary
The purpose of this study is to develop a highly sensitive method for assessing the functional activity of platelets for use in the differential diagnosis of thrombocytopenia and platelet defects.
Detailed Description
The purpose of this study is to develop a highly sensitive method for assessing the functional activity of platelets using flow cytometry and turbidimetric and impedance aggregometry for use in the differential diagnosis of thrombocytopenia and platelet defects
Investigators
Eligibility Criteria
Inclusion Criteria
- •Pediatric participants with hemorrhagic syndrome (hemorrhagic rash, bruising, bleeding, increased bleeding of the skin and mucous membranes, spontaneous hemorrhages in soft tissues and joints)
- •Pediatric participants with acute leukemia
- •Parameters of the plasma level of blood coagulation correspond to the age normal levels
Exclusion Criteria
- •Anticoagulation drug therapy
- •Antiaggregation drug therapy
- •Biologically active additives
Outcomes
Primary Outcomes
Expression of CD41 by platelets
Time Frame: baseline
Assessment of platelet functional activity by the detection of CD41
Impedance of platelet aggregates
Time Frame: baseline
Assessment of platelet functional activity by impedance aggregometry
Fibrinogen level
Time Frame: baseline
Assessment of blood coagulation using Fibrinogen level test
Expression of CD42b by platelets
Time Frame: baseline
Assessment of platelet functional activity by the detection of CD42b
Expression of CD62p by platelets
Time Frame: baseline
Assessment of platelet functional activity by the detection of CD62p
Level of platelet aggregation
Time Frame: baseline
Assessment of platelet functional activity by turbidimetric aggregometry
Expression of Annexin V by platelets
Time Frame: baseline
Assessment of platelet functional activity by the detection of Annexin V
aPTT
Time Frame: baseline
Assessment of blood coagulation using aPTT
Expression of PAC-1 by platelets
Time Frame: baseline
Assessment of platelet functional activity by the detection of PAC-1
Expression of CD63 by platelets
Time Frame: baseline
Assessment of platelet functional activity by the detection of CD63
Prothrombin test
Time Frame: baseline
Assessment of blood coagulation using Prothrombin test
Thrombin time
Time Frame: baseline
Assessment of blood coagulation using Thrombin time test