Development of a Method for Assessing the Functional Activity of Platelets for the Differential Diagnosis of Thrombocytopathies Using Flow Cytometry
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 200
- 主要终点
- Expression of CD41 by platelets
研究概览
简要总结
The purpose of this study is to develop a highly sensitive method for assessing the functional activity of platelets for use in the differential diagnosis of thrombocytopenia and platelet defects.
详细描述
The purpose of this study is to develop a highly sensitive method for assessing the functional activity of platelets using flow cytometry and turbidimetric and impedance aggregometry for use in the differential diagnosis of thrombocytopenia and platelet defects
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 1 Year 至 17 Years（Child）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Pediatric participants with hemorrhagic syndrome (hemorrhagic rash, bruising, bleeding, increased bleeding of the skin and mucous membranes, spontaneous hemorrhages in soft tissues and joints)
- •Pediatric participants with acute leukemia
- •Parameters of the plasma level of blood coagulation correspond to the age normal levels
排除标准
- •Anticoagulation drug therapy
- •Antiaggregation drug therapy
- •Biologically active additives
结局指标
主要结局
Expression of CD41 by platelets
时间窗: baseline
Assessment of platelet functional activity by the detection of CD41
Impedance of platelet aggregates
时间窗: baseline
Assessment of platelet functional activity by impedance aggregometry
Fibrinogen level
时间窗: baseline
Assessment of blood coagulation using Fibrinogen level test
Expression of CD42b by platelets
时间窗: baseline
Assessment of platelet functional activity by the detection of CD42b
Expression of CD62p by platelets
时间窗: baseline
Assessment of platelet functional activity by the detection of CD62p
Level of platelet aggregation
时间窗: baseline
Assessment of platelet functional activity by turbidimetric aggregometry
Expression of Annexin V by platelets
时间窗: baseline
Assessment of platelet functional activity by the detection of Annexin V
aPTT
时间窗: baseline
Assessment of blood coagulation using aPTT
Expression of PAC-1 by platelets
时间窗: baseline
Assessment of platelet functional activity by the detection of PAC-1
Expression of CD63 by platelets
时间窗: baseline
Assessment of platelet functional activity by the detection of CD63
Prothrombin test
时间窗: baseline
Assessment of blood coagulation using Prothrombin test
Thrombin time
时间窗: baseline
Assessment of blood coagulation using Thrombin time test
次要结局
未报告次要终点