Molecular Taxonomy of Surgically-harvested Ocular Tissues Defined by Single-cell Transcriptomics

Not Applicable

• Conditions: Glaucoma; Diabetic Retinopathy; Proliferative Vitreoretinopathy; Pterygium
• Interventions: Procedure: ocular surgery

• Registration Number: NCT04682054
• Lead Sponsor: The First Affiliated Hospital with Nanjing Medical University

Brief Summary

The surgically-harvested eye tissue (ie. vitreous-retinal proliferative membrane, outflow pathway, retinas, and pterygium) is a complex tissue responsible for maintaining intraocular homeostasis or mediating ocular pathogenesis. Dysfunction of one or more resident cell types within the tissues results in different ocular disorder, leading to vision loss, or even blindness. In this study, we aim to use single-cell RNA sequencing to generate a comprehensive cell atlas of surgically-harvested eye tissues.

Detailed Description

Not available

Study Timeline

• Status Verified: 2020-12
• Study First Submitted: 2020-12-07
• Study First Submitted QC: 2020-12-20
• Last Update Submitted: 2020-12-20
• Study First Posted: 2020-12-23
• Last Update Posted: 2020-12-23
• Study Start: 2021-01-15
• Primary Completion: 2021-12-31
• Study Completion: 2021-12-31

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 80

Inclusion Criteria

1. Patients with ocular diseases and require surgery intervention.
2. The surgery intervention remove pathological tissue during surgery.
3. Patients consent to the treatment and single-cell sequencing.

Exclusion Criteria

1. Surgically removed eye tissue cannot meet with the quality control of single-cell sequencing (mainly the tissue weight and cell counts);

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
surgically-removed eye tissue	ocular surgery	-

Primary Outcome Measures

Name	Time	Method
counts of each cell type	1 month	single-cell sequencing identify what cells and the counts within the eye tissue

Trial Locations

Locations (1)

First Affiliated Hospital of Nanjing Medical University; 🇨🇳 Nanjing, Jiangsu, China