NCT04682054UnknownNot Applicable
Molecular Taxonomy of Surgically-harvested Ocular Tissues Defined by Single-cell Transcriptomics
The First Affiliated Hospital with Nanjing Medical University1 site in 1 country80 target enrollmentStarted: January 15, 2021Last updated:
Conditions
Trial Snapshot
- Phase
- Not Applicable
- Enrollment
- 80
- Locations
- 1
- Primary Endpoint
- counts of each cell type
Study Overview
Brief Summary
The surgically-harvested eye tissue (ie. vitreous-retinal proliferative membrane, outflow pathway, retinas, and pterygium) is a complex tissue responsible for maintaining intraocular homeostasis or mediating ocular pathogenesis. Dysfunction of one or more resident cell types within the tissues results in different ocular disorder, leading to vision loss, or even blindness. In this study, we aim to use single-cell RNA sequencing to generate a comprehensive cell atlas of surgically-harvested eye tissues.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 90 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Patients with ocular diseases and require surgery intervention.
- •The surgery intervention remove pathological tissue during surgery.
- •Patients consent to the treatment and single-cell sequencing.
Exclusion Criteria
- •Surgically removed eye tissue cannot meet with the quality control of single-cell sequencing (mainly the tissue weight and cell counts);
Outcomes
Primary Outcomes
counts of each cell type
Time Frame: 1 month
single-cell sequencing identify what cells and the counts within the eye tissue
Secondary Outcomes
No secondary outcomes reported
Investigators
Study Sites (1)
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