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Effect of Orange Juice and Healthy Diet on Cardiometabolic Risk Factors of Individuals With Metabolic Syndrome

Not Applicable
Completed
Conditions
Metabolic Syndrome
Interventions
Other: Orange Juice (500 mL/d)
Registration Number
NCT03301675
Lead Sponsor
São Paulo State University
Brief Summary

This study aimed to verify if combination of a healthy diet and orange juice consumption can minimize cardiometabolic risk factors for Metabolic Syndrome (MetS)

Detailed Description

The clinical study was parallel, controlled, and randomized with metabolic syndrome subjects (ATPIII, AHA / NHLA) aimed at the consumption of an energy-balanced balanced diet for 12 weeks and divided into two groups: Control (n = 38): dietary guidance only; and Orange Juice (n = 38): diet guidance associated with 500 mL / day of 100% whole orange juice. The recruitment process began in June 2016, the intervention was carried out from September 2016 to December 2016, and the data analysis started in January 2016. The sample number took into account variances on LDL-C, with a type I error α = 0.05 and a type II error β = 0.2 (80% power). The minimum sample size should have 32 individuals per group (n = 64). Considering an approximately 15% dropout rate, the final sample size of study was constituted by 38 individuals per group. Primary and secondary endpoints were the reduction of LDL-C and modification of the levels of cardiometabolic risk factors, inflammatory and hemodynamics parameters, respectively. Kolmogorov Smirnov and Levene test assessed normality and homogeneity of data, respectively. T-test was conducted to identify possible differences between OJ and control groups at baseline. A linear mixed-effects model was apply to determine the time effect within and between groups (Sidak post hoc) and P significance was set up ≤ 0.05. The assessment of body composition, metabolic biomarkers and food intake were analyzed over a 12-week intervention.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
76
Inclusion Criteria
  • Three or more of the risk factors of MS: (1) waist circumference man ≥ 102 cm and woman ≥ 88 cm; (2) triglycerides ≥ 150 mg / dL; (3) HDL-C man ≤ 40 mg / dL and woman ≤ 50 mg / dL; (4) blood pressure ≥ 130 / ≥ 85 mm Hg and (5) fasting glucose ≥ 100 mg / dL (common diabetes, high blood pressure);
  • 25 ≥ BMI ≤ 39.9 kg / m - overweight to grade II obesity;
  • Like to consume orange juice;
Exclusion Criteria
  • Pregnant / nursing;
  • Use of vitamins or vitamin-food supplements in the last three months;
  • Individuals with diseases that require specific diet recommendations such as diabetes mellitus with insulin therapy and carbohydrate counts, cancer, chronic liver and kidney disease.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Orange juiceOrange Juice (500 mL/d)Orange Juice: Thirty-eight individuals with MetS were submitted to a healthy diet (energy was based on individual actual weight) plus 100% orange juice (500 mL/d) during 12 weeks.
Primary Outcome Measures
NameTimeMethod
LDL-C12 weeks

mg/dL

Secondary Outcome Measures
NameTimeMethod
TNF-alfa12 weeks

pg/ml

Cardiovascular risk index12 weeks

% risk

HDL-C12 weeks

mg/dL

Glucose12 weeks

mg/dL

Waist circunference12 weeks

cm

Body fat mass12 weeks

kg

Insulin12 weeks

µU/mL

hsCRP12 weeks

mg/dL

IL-612 weeks

pg/ml

Triglycerides12 weeks

mg/dL

Blood pressure systolic and diastolic12 weeks

mm Hg

Body lean mass12 weeks

kg

Visceral fat area12 weeks

kg

Total cholesterol12 weeks

mg/dL

Body fat12 weeks

percentage

ICAM12 weeks

ng/mL

VCAM12 weeks

ng/mL

Flow-mediated dilatation of the brachial artery - BA-FMD12 weeks

percentage

Common carotid artery intima-media thickness - CCA-IMT12 weeks

mm

Pulse wave velocity - PWV12 weeks

cm/s

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