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Molecular Imaging of HER2 Expression in Breast Cancer Using [99mTc]Tc-G3-(G3S)3C Before Chemo/Targeted Therapy

Not Applicable
Completed
Conditions
Breast Cancer Female
Interventions
Registration Number
NCT05923268
Lead Sponsor
Tomsk National Research Medical Center of the Russian Academy of Sciences
Brief Summary

The study should evaluate distribution of \[99mTc\]Tc-G3-(G3S)3C in patients with primary HER2-positive and HER2-negative breast cancer

Detailed Description

Overall goal of the project: To determine HER2 expression level in primary breast cancer before neoadjuvant system therapy (chemotherapy or/and targeted therapy).

Phase I. Distribution of \[99mTc\]Tc-G3-(G3S)3C in patients with primary breast cancer. The study should evaluate distribution of \[99mTc\]Tc-G3-(G3S)3C in patients with primary HER2-positive and HER2-negative breast cancer.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
10
Inclusion Criteria
  1. Subject is > 18 years of age

  2. Availability of results from HER2 status previously determined on material from the primary tumor and metastatic LN, either a. HER2-positive, defined as a DAKO HercepTest™ score of 3+ or FISH positive or b. HER2-negative, defined as a DAKO HercepTest™ score of 0 or 1+; or else if 2+ then FISH negative

  3. Hematological, liver and renal function test results within the following limits:

    • White blood cell count: > 2.0 x 109/L
    • Hemoglobin: > 80 g/L
    • Platelets: > 50.0 x 109/L
    • ALT, ALP, AST: =< 5.0 times Upper Limit of Normal
    • Bilirubin =< 2.0 times Upper Limit of Normal
    • Serum creatinine: Within Normal Limits
  4. A negative pregnancy test for all patients of childbearing potential. Sexually active women of childbearing potential participating in the study must use a medically acceptable form of contraception for at least 30 days after study termination

  5. Subject is capable to undergo the diagnostic investigations to be performed in the study

  6. Informed consent

Exclusion Criteria
  1. Any system therapy (chemo-/targeted therapy)
  2. Second, non-breast malignancy
  3. Active current autoimmune disease or history of autoimmune disease
  4. Active infection or history of severe infection within the previous 3 months (if clinically relevant at screening) 4. Known HIV positive or chronically active hepatitis B or C
  5. Administration of other investigational medicinal product within 30 days of screening
  6. Ongoing toxicity > grade 2 from previous standard or investigational therapies, according to US National Cancer Institute's

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
The tested injected doses of [99mTc]Tc-G3-(G3S)3C 3000 μg[99mTc]Tc-G3-(G3S)3CAt least five (5) evaluable subjects with HER2-positive status and at least five (5) subjects with HER2-negative status have to be enrolled in the study for each tested protein dose. The tested injected dose 3000 μg. Subjects withdrawn from the study for any reason will be replaced.
Primary Outcome Measures
NameTimeMethod
SPECT-based [99mTc]Tc-G3-(G3S)3C background uptake value (counts)6 hours

Focal uptake of \[99mTc\]Tc-G3-(G3S)3C in the regions without pathological findings will be assessed with SPECT and measured in counts

Tumor-to-background ratio (SPECT)6 hours

The SPECT-based tumor-to-background ratio will be calculated as follows: the value of \[99mTc\]Tc-G3-(G3S)3C uptake coinciding with tumor lesions (counts) will be divided by the value of \[99mTc\]Tc-G3-(G3S)3C uptake coinciding with the regions without pathological findings (counts)

Gamma camera-based whole-body [99mTc]Tc-G3-(G3S)3C uptake value (%)6 hours

Whole-body \[99mTc\]Tc-G3-(G3S)3C uptake coinciding with normal organs and tissues will be assessed using gamma camera and calculated as percentage (%) of the injected dose of the radiopharmaceutical

SPECT-based [99mTc]Tc-G3-(G3S)3C uptake value in tumor lesions (counts) [99mTc]Tc-G3-(G3S)3C uptake coinciding with tumor lesions will be assessed using single-photon emission computed tomography and measured in counts6 hours

SPECT-based \[99mTc\]Tc-G3-(G3S)3C uptake value in tumor lesions (counts) \[99mTc\]Tc-G3-(G3S)3C uptake coinciding with tumor lesions will be assessed using single-photon emission computed tomography and measured in counts

Secondary Outcome Measures
NameTimeMethod
Safety attributable to [99mTc]Tc-G3-(G3S)3C injections (vital signs) (% of cases with abnormal findings relative to baseline)24 hors

The safety attributable to \[99mTc\]Tc-G3-(G3S)3C injections will be evaluated based on the assessments of vital signs (% of cases with abnormal findings relative to baseline)

Safety attributable to [99mTc]Tc-G3-(G3S)3C injections (physical examination) (% of cases with abnormal findings relative to baseline)24 hours

The safety attributable to \[99mTc\]Tc-G3-(G3S)3C injections will be evaluated based on the assessments of physical examination (% of cases with abnormal findings relative to baseline)

Safety attributable to [99mTc]Tc-G3-(G3S)3C injections (% of incidence and severity of adverse events)24 hours

The safety attributable to \[99mTc\]Tc-G3-(G3S)3C injections will be evaluated based on the rate of adverse events (%)

Safety attributable to [99mTc]Tc-G3-(G3S)3C injections (ECG ) (% of cases with abnormal findings relative to baseline)24 hours

The safety attributable to \[99mTc\]Tc-G3-(G3S)3C injections will be evaluated based on the assessments of ECG (% of cases with abnormal findings relative to baseline)

Safety attributable to [99mTc]Tc-G3-(G3S)3C injections (laboratory tests) (% of cases with abnormal findings relative to baseline)Time Frame: 24 hours

The safety attributable to \[99mTc\]Tc-G3-(G3S)3C injections will be evaluated based on the blood and urine laboratory tests (% of cases with abnormal findings relative to baseline)

Safety attributable to [99mTc]Tc-G3-(G3S)3C injections (% concomitant medication)24 hours

The safety attributable to \[99mTc\]Tc-G3-(G3S)3C injections will be evaluated based on the rate of administration of concomitant medication (%)

Trial Locations

Locations (1)

Olga

🇷🇺

Tomsk, Russian Federation

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