Serological Evaluation of 77A/G and 11A/12A Gene of MMP-13 in Periodontitis Affected and Healthy Individuals

• Conditions: Generalized Adult Periodontitis
• Interventions: Genetic: blood samples collected from all patients

• Registration Number: NCT02423304
• Lead Sponsor: Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre

Brief Summary

50 periodontitis 50 healthy individuals serological evaluation will done to see association of 77A/G AND 11A/12A gene polymorphisms of MMP-13

Detailed Description

* The study will be conducted on 100 individuals

* 50 chronic periodontitis and 50 healthy individuals will be recruited from the Out Patient Department of Periodontics in the study.

* An informed consent will be obtained from patients who wish to participate in the study.

* Patients will be categorized by Periodontal Disease Index (PDI) by P. Ramfjord(1959)

* After clinical examination, blood samples will be collected and assessed for MMP 13 Gene Polymorphisms.

* Subjects will be selected according to the study criteria and divided into two groups (50 each).

1. Test group

2. Control group

Study Timeline

• Status Verified: 2015-04
• Study First Submitted: 2015-04-17
• Study First Submitted QC: 2015-04-21
• Last Update Submitted: 2015-04-21
• Study First Posted: 2015-04-22
• Last Update Posted: 2015-04-22
• Study Start: 2015-05
• Primary Completion: 2016-05
• Study Completion: 2016-06

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Test group

1. Patients with chronic periodontitis classified according to American Academy of Periodontology ( AAP) 1999

2. Subjects aged between 30-55 years

   • Control group Systemically and periodontally healthy individuals of same age group

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Exclusion Criteria

1. Present and past Smokers
2. Presence of any systemic diseases.
3. Oral diseases other than Periodontitis
4. With a previous history of periodontal treatment in the past 6 months and subjects under any medications.
5. Family history of any diagnosed genetic disorders.

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Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
test	blood samples collected from all patients	50 periodontitis patients • Patients will be categorized by Periodontal Disease Index (PDI) by P. Ramfjord(1959) blood samples collected from all patients serum separated and evaluated for77A/G AND 11A/12A associated gene polymorphism of Matrix Metallo Proteinase( MMP)-13 in all the patients. studies showing that there is co relation of these genes with increase inflammation.
control	blood samples collected from all patients	50 periodontally healthy individuals blood samples collected from all patients serum separated and evaluated for77A/G AND 11A/12A associated gene polymorphism of Matrix Metallo Proteinase (MMP)-13 in all the patients

Primary Outcome Measures

Name	Time	Method
to evaluate the association of 77A/G AND 11A/12A gene polymorphism of mmp-13 in periodontitis effected and healthy individuals	6 months	collect DNA samples from serum and evaluate for gene polymorphism