The Effect of Juice Types on the Responses to Air Pollution

Not Applicable

Completed

• Conditions: Air Pollution
• Interventions: Dietary Supplement: Beet juice; Dietary Supplement: Orange juice

• Registration Number: NCT02027415
• Lead Sponsor: Rutgers, The State University of New Jersey

Brief Summary

The study hypothesis is that drinking different juices will affect the body's responses to air pollution. Subjects will be exposed to air pollution during a 2-hour car ride on the NJ (New Jersey) Turnpike. Each subject will be asked to do this twice. Before one car ride, the subject will be asked to drink orange juice. Before the other car ride, the subject will be asked to drink beet juice. Samples of blood and exhaled breath will be collected before, immediately after, and 24 hours after each car ride. Levels of nitrites/nitrates will be measured in the blood and breath.

Detailed Description

Subjects will participate in two exposure sessions. The air pollution exposure consists of a 2-hour car ride along the NJ Turnpike. Samples will be collected at 3 time points for each exposure session: pre-exposure, immediately post-exposure, and 24 hours post-exposure. Blood samples will be collected immediately before and after a 5-minute occlusion at each time point. The subject will also be asked to provide samples of exhaled breath and complete symptom and stress questionnaires at each time point.

Blood samples will be analyzed for serum nitrite. Exhaled breath samples will be analyzed for nitrates and nitrites.

Study Timeline

• Study Start: 2013-12
• Study First Submitted: 2013-12-16
• Study First Submitted QC: 2014-01-02
• Study First Posted: 2014-01-06
• Primary Completion: 2014-10
• Study Completion: 2014-10
• Status Verified: 2017-12
• Last Update Submitted: 2017-12-11
• Last Update Posted: 2017-12-13

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• Healthy
• Must live within 20 miles of Piscataway, NJ

Exclusion Criteria

• • Are a current smoker

  • Are pregnant or breastfeeding
  • Are HIV-positive
  • Have an active medical condition such as high blood pressure, asthma, diabetes or other serious disease
  • Have a heart condition such as atrial flutter, atrial fibrillation or a pacemaker
  • Are unable to ride comfortably in a car for 2 hours
  • Travel to the EOHSI (Environmental and Occupational Health Sciences Institute) Clinic Center on major highways for more than 3 miles (the Garden State Parkway, the New Jersey Turnpike, Routes 287, 1, 18, or 9).

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Beet Orange	Beet juice	Beet juice will be given prior to the first car ride and orange juice will be given prior to the second car ride.
Orange Beet	Beet juice	Orange juice will be given before the first car ride and beet juice will be given before the second car ride.
Beet Orange	Orange juice	Beet juice will be given prior to the first car ride and orange juice will be given prior to the second car ride.
Orange Beet	Orange juice	Orange juice will be given before the first car ride and beet juice will be given before the second car ride.

Primary Outcome Measures

Name	Time	Method
Change in plasma nitrite concentration	Prior to the car ride, immediately after the 2-hour car ride, and 24 hours later	Change in plasma nitrite concentration pre- and post-occlusion

Secondary Outcome Measures

Name	Time	Method
Change in nitrite/nitrate concentration in exhaled breath	Prior to the car ride, immediately after the 2 hour car ride and 24 hours later	Change in nitrite/nitrate concentration in exhaled breath

Trial Locations

Locations (1)

Environmental and Occupational Health Sciences Institute; 🇺🇸 Piscataway, New Jersey, United States