Cryosurvival of Embryos From Dysmorphic Oocytes
Completed
- Conditions
- CryopreservationBlastocyst
- Interventions
- Procedure: Cryopreservation, thawing and blastocyst culture
- Registration Number
- NCT00521443
- Lead Sponsor
- V.K.V. American Hospital, Istanbul
- Brief Summary
Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- Not specified
Inclusion Criteria
- Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure.
- Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle.
Exclusion Criteria
- Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture.
- Embryos obtained from abnormally fertilized zygotes.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Normal Cryopreservation, thawing and blastocyst culture Embryos derived from morphologically normal MII oocytes Irregular Shapes Cryopreservation, thawing and blastocyst culture Embryos derived from irregularly shaped oocytes. Large PVS Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with large perivitelline space. Dark Zona Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with dark zona pellucida Dark cytoplasm Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with dark cytoplasm Vacuolar cytoplasm Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with vacuolated cytoplasm Central granulation Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with centrally granulated cytoplasm Double extra Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with double extracytoplasmic abnormalities Double combined Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly Double cytoplasmic Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with any two cytoplasmic anomalies Triple extra Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with triple extracytoplasmic anomaly Triple combined Cryopreservation, thawing and blastocyst culture Embryos derived from oocytes with triple combined anomalies
- Primary Outcome Measures
Name Time Method
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
VKV American Hospital
🇹🇷Istanbul, Turkey