Molecular and Structural Comparison of Hypospadic and Normal Children Prepuces
- Conditions
- Hypospadias
- Registration Number
- NCT03838458
- Lead Sponsor
- Trakya University
- Brief Summary
The aim of this study is to compare prepuce tissue samples from healthy male children who applied for circumcision and children with isolated hypospadias. Estrogen receptor 1 (ER1), androgen receptor (AR), FGFR2, transforming growth factor beta (TGF beta) and HOXA13 gene expression in the prepuce is planned to investigate via polymerase chain reaction (PCR) method. Mucosal vascular and nerve densities, and extracellular matrix density of laminin and collagen protein is planned to investigate via immunohistochemical examination.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 52
- To apply for circumcision, not to have any genitourinary abnormalities/conditions for control group.
- To have isolated hypospadias for study group.
- To have genitourinary surgery before.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Genetic analysis 1 1 year Androgen receptor (AR) gene PCR analysis
Genetic analysis 3 1 year FGFR2 gene PCR analysis
Genetic analysis 2 1 year Estrogen receptor 1 (ER1) gene PCR analysis
Immunohistochemical study 2 1 year Mucosal nerve density is planned to determine via PGP 9.5
Genetic analysis 5 1 year HOXA13 gene PCR analysis
Histological study 2 1 year Collagen is planned to determine in extracellular matrix via light microscope
Genetic analysis 4 1 year TGF beta gene PCR analysis
Histological study 1 1 year Laminin is planned to determine in extracellular matrix via light microscope
Immunohistochemical study 1 1 year Mucosal vessel density is planned to determine via CD34
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Trakya University Faculty of Medicine
🇹🇷Edirne, Turkey