Detection of Thyrotrophin Receptor in Human Myometrium
- Conditions
- Thyrothrophin Receptor in Myometrium Tissue
- Interventions
- Procedure: Myometrial biopsy
- Registration Number
- NCT01348191
- Lead Sponsor
- Catharina Ziekenhuis Eindhoven
- Brief Summary
It has been recognized for many decades that high thyrotrophin (TSH) levels in pregnant women are associated with poor obstetric outcome. Also, there is evidence that high TSH is related to fetal position at term, including breech which in turn is associated with obstetric complications.
However, the mechanism behind remains to be elucidated.
The current project is of basic-fundamental nature. It is used to better understand basis physiological processes. As in many other studies of similar basic nature, few numbers are always included.
If a TSH receptor will be detected, future randomized controlled trials (RCT) might be worthwhile with large numbers of women who will be treated with thyroxine to possibly prevent abnormal fetal position during normal pregnancy.
The aim of the current study is to evaluate whether a TSH receptor can be demonstrated in human myometrium. If so, the pathophysiology of high TSH in relation to obstetric outcome will become more clear. Furthermore, the aim is to test the myometrium in vitro for its relaxation and contractility and for the conductance of electropotentials.
- Detailed Description
It has been recognized for many decades that high thyrotrophin (TSH) levels in pregnant women are associated with poor obstetric outcome. Also, there is evidence that high TSH is related to fetal position at term, including breech which in turn is associated with obstetric complications.
However, the mechanism behind remains to be elucidated. It has been shown that high TSH affects relaxation and contraction of the smooth muscle in large blood vessels in human. In animals, there is some evidence that TSH interferes with uterine contractility. Although a TSH receptor has been demonstrated outside the thyroid in bone, brain and heart, so far no research on a possible TSH receptor in human uterine tissue has been published.
The current study is a pilot study in which in a limited number of participants (pregnant women, n=10) during elective Caesarean section a uterine specimen will be collected for analysis in a immune-laboratory.
Another part of the specimen will be analysed in a technical laboratory(Technical University of Eindhoven)for analysing the relaxation and contractility of the myometrium and for studying the conductance of electropotentials.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- 10
- Elective caesarean section
- Term pregnancy > 37 weeks
- Age > 18 years
- Previous caesarean scar
- Gestational age < 37 weeks
- Maternal temperature > 37.8 degrees Celsius
- Meconium stained liquor
- Foetal distress
- Maternal diabetes
- Seropositivity
- Use of thyroid medication
- Maternal thyroid disease
- Age < 18 years
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Elective caesarean section Myometrial biopsy Population: ten pregnant women, scheduled for elective caesarean section with a term pregnancy ( \> 37 weeks). Inclusion criteria * Elective caesarean section * Term pregnancy \> 37 weeks * Age \> 18 years Exclusion criteria * Previous caesarean scar * Gestational age \< 37 weeks * Maternal temperature \> 37.8 degrees Celsius * Meconium stained liquor * Foetal distress * Maternal diabetes * Seropositivity * Use of thyroid medication * Maternal thyroid disease * Age \< 18 years
- Primary Outcome Measures
Name Time Method Thyrothrophin receptor three months Immunodetection of Thyrothrophin receptor in myometrium tissue
- Secondary Outcome Measures
Name Time Method electropotentials in myometrium and relaxation and contractility Three months Investigation of relaxation and contractility of myometrium. Conductance of electropotentials of myometrium tissue
Trial Locations
- Locations (4)
Erasmus MC
🇳🇱Rotterdam, Zuid-Holland, Netherlands
University of Tilburg
🇳🇱Tilburg, Brabant, Netherlands
Catharina-hospital
🇳🇱Eindhoven, Brabant, Netherlands
Eindhoven University of Technology
🇳🇱Eindhoven, Brabant, Netherlands