Growth of skeletal muscle in response to feeding specific amino acids
Not Applicable
Completed
- Conditions
- Resistance exerciseNot Applicable
- Registration Number
- ISRCTN98737111
- Lead Sponsor
- GlaxoSmithKline Nutritional Healthcare
- Brief Summary
2017 results in: https://www.ncbi.nlm.nih.gov/pubmed/28638350 (added 31/10/2019)
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Male
- Target Recruitment
- 10
Inclusion Criteria
1. Male, aged 18-45 years
2. Healthy (no known metabolic disorder)
3. Experienced weight lifter for more than 6 months (at least 2 leg resistance training sessions per week)
Exclusion Criteria
Not simultaneously taking part in another scientific/clinical study
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <br> Fractional synthetic rate (FSR), measured using gas combustion isotope ratio chromatography mass spectrometry (GCCIRMS) over the 4h period following drink ingestion.<br>
- Secondary Outcome Measures
Name Time Method <br> 1. Signalling proteins, measured using western blots<br> 2. Amino acids in blood, measured using gas chromatography mass spectrometry (GCMS)<br> 3. Tracers enrichment in muscle, measured using GCMS<br> 4. Tracers enrichment in plasma, measured using GCMS<br> 5. Insulin in serum, measured using enzyme-linked immunosorbent assay (ELISA)<br> 6. Glucose in plasma, measured using automated analysers<br><br> Blood sample timepoints: -240, -145, -85, -25, 0, 15, 30, 45, 60, 75, 90, 120, 150, 180, 240 min<br> Muscle biopsy timepoints: 0, 60 and 240 min<br>