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Primary Liver Cancer Early Detection

Recruiting
Conditions
Liver Cancer
Interventions
Device: Liver-cancer early detection test
Registration Number
NCT05996666
Lead Sponsor
The First Hospital of Jilin University
Brief Summary

In the recently published multi-center, prospective, single-blind study (THUNDER study), using the methylation signal in cfDNA isolated from the peripheral blood to detect the six types of cancer, the sensitivity for liver cancer detection achieved 87.8%, with a specificity of 98.9%. In this study, a multicenter, case-control study is designed to establish an early cancer detection model based on cfDNA methylation biomarkers using qPCR to detect primary liver cancer and further validate the performance of the model.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
701
Inclusion Criteria

Inclusion Criteria for all participants

  • Individuals aged 18-74 years old
  • Individuals capable of giving signed and legally effective informed consent voluntarily

Inclusion Criteria for liver cancer participants:

  • Individuals newly diagnosed with or suspected of having liver cancer (including hepatocellular carcinoma [HCC], intrahepatic cholangiocarcinoma [ICC], and combined hepatocellular-cholangiocarcinoma [cHCC-CCA]).
  • Individuals without any anti-cancer therapy prior to blood sample collection.

Inclusion Criteria for participants with benign liver diseases:

  • Individuals newly diagnosed as benign liver diseases before blood sample collection
  • Individuals without curative treatment for the disease before blood sample collection

Inclusion Criteria for participants with interfering cancers:

  • Individuals diagnosed with or suspected of having interfering cancer
  • Individuals without any anti-cancer therapy prior to blood sample collection

Inclusion Criteria for healthy participants:

  • No cancer-related or other clinical symptoms 30 days prior to blood sample collection
  • No prior history of benign liver diseases
Exclusion Criteria

Exclusion Criteria for All the Participants:

  • Individuals cannot provide blood samples at the study-specified blood collection sites
  • Pregnancy or lactating women
  • Recipients of organ transplant or prior non-autologous (allogeneic) bone marrow transplant or stem cell transplant
  • Recipients of blood transfusion within 7 days prior to blood sample collection
  • Recipients of anti-infective treatment within 14 days prior to blood sample collection
  • Recipients of any anti-cancer therapy within 30 days prior to study blood draw, due to diseases other than cancer
  • Individuals with a history of malignant tumor

Exclusion Criteria for liver cancer participants:

  • Known prior or current diagnosis of other types of malignancies or multiple primary cancers
  • No confirmed diagnosis of liver cancer after blood sample collection

Exclusion Criteria for participants with benign liver diseases:

  • Individuals with a confirmed diagnosis of a malignant tumor or precancerous lesion
  • Individuals who cannot be diagnosed as having benign liver diseases after blood sample collection

Exclusion Criteria for participants with interfering cancers:

  • Known prior or current diagnosis of other types of malignancies or multiple primary cancers
  • No confirmed diagnosis of any type of interfering cancers after blood sample collection

Exclusion Criteria for healthy participants:

  • Prior or ongoing treatment of cancers within 3 years prior to blood sample collection
  • Clinically significant or uncontrolled comorbidities

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Interfering cancer armLiver-cancer early detection testParticipants with new diagnosis of interfering cancer types, from whom blood samples will be collected.
Healthy armLiver-cancer early detection testParticipants without known presence of malignancies or benign diseases of liver, from whom blood samples will be collected.
Benign diseases armLiver-cancer early detection testParticipants with benign diseases of cancer, from whom blood samples will be collected.
Liver cancer armLiver-cancer early detection testParticipants with new diagnosis of liver cancer, from whom blood samples will be collected.
Primary Outcome Measures
NameTimeMethod
Performance of liver cancer early detection model based on selected cfDNA methylation markers using qPCR2023.07.01-2023.12.30

Sensitivity and specificity of liver cancer detection model based on selected cfDNA methylation biomarkers using qPCR in subjects with primary liver cancer or benign liver diseases, interfering cancers and healthy individuals

Secondary Outcome Measures
NameTimeMethod
Comparison of currently used biomarkers for liver cancer detection2023.07.01-2023.12.30

Comparison of the sensitivity and specificity of the liver cancer early detection model and other biomarkers of liver cancer in detecting early liver cancer

Performance of the liver cancer early detection model in detecting liver cancer at early stage2023.07.01-2023.12.30

Sensitivity of the liver cancer early detection model in patients with small liver cancer lesions or AFP negative

Performance of liver cancer early detection model in detecting liver cancer at different stages and subtypes2023.07.01-2023.12.30

Sensitivity of the liver cancer early detection model in different stages or different pathological subtypes

Performance of liver cancer early detection model to differentiate non-liver cancers2023.07.01-2023.12.30

Specificity of the liver cancer early detection model to differentiate patients with benign liver diseases or different interfering cancer types from liver cancer

Trial Locations

Locations (2)

Jilin university first hospital

🇨🇳

Changchun, Jilin, China

Eastern Hepatobiliary Surgery Hospital

🇨🇳

Shanghai, Shanghai, China

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