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A Cohort of Patients With Cystinosis : Compliance to Cysteamine and Neurological Complications

Not Applicable
Completed
Conditions
Cystinosis
Interventions
Other: Cysteamine bitartrate
Registration Number
NCT02012114
Lead Sponsor
Hospices Civils de Lyon
Brief Summary

Preventing late onset of Cystinosis such as neurological complications and improving compliance to cysteamine treatment remain major challenges in management of subjects with cystinosis.

This study is designed to describe the relationship between compliance of patients with cystinosis treated with cysteamine and treatment efficacy and to understand the pathophysiologic mechanism of neurological disorders. Is cysteamine crossing the blood brain barrier? What is the impact of cystine accumulation in Cerebro Spinal Fluid and Central Nervous System? Our Primary objective is to study the relationship between compliance of patients treated with cysteamine and the WBC cystine level.

Secondary, the study will assess relationship between compliance to cysteamine and its neurological consequences.

The expected duration of the study is 48 months. The enrolment period is 24 months and the study participation of each subject is 24 months. Eligible participants are male and female (age \> 4 years) with confirmed diagnosis of cystinosis and receiving any oral cysteamine treatment: Cystagon or RP103.

The compliance under cysteamine is measured using electronic devices, accountability of study treatment, and information in patients' diary. Specific memory and visuoperceptual tests are performed at the beginning and at the end of patients'participation. Nuclear Magnetic Resonance spectroscopy is used to detect possible sites of cystine accumulation in the CNS and their relationship with compliance to cysteamine treatment. NMRS is also used to establish a relationship with the neuropsychological status of the subject.

To describe absorption, distribution and elimination of cysteamine, and its metabolic pathways, and to determine the concentration effect and dose effect relationship, blood samples are performed at each study visit. A lumbar puncture is also proposed to participants to verify if cysteamine is crossing the blood brain barrier. New tools are used to compare metabonomic networks in patients with cystinosis and their controls.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
65
Inclusion Criteria
  • Age and sex matched to study population: Age range: patients aged 4 to 5 years, 6 to 11 years, patients aged 12 to 17 years, adult patients: 18 or above.

Sex: male or female

  • Subjects or their parent or legal guardian must provide written informed consent prior to participation in the study.
  • Subjects covered by or having the right to social security.
Exclusion Criteria
  • Any uropathology or nephropathology.

CONTROLS FOR NMRS ASSESSMENTS

Inclusion Criteria:

  • Age and sex matched to study population: Age range: patients aged 4 to 5 years, 6 to 11 years, patients aged 12 to 17 years, adult patients: 18 or above.

Sex: male or female

  • Subjects or their parent or legal guardian must provide written informed consent prior to participation in the study.
  • Subjects covered by or having the right to social security.

Exclusion Criteria:

  • Any uropathology or nephropathology.
  • Any neurological and/or psychiatric disorder
  • Contra-indication to MRI assessment.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Cysteamine BitartrateCysteamine bitartrateSingle arm study. Subjects receive their current oral form of cysteamine bitartrate treatment : Cystagon® or RP103
Primary Outcome Measures
NameTimeMethod
WBC cystine levelsDay1and every three months : Month1, Month3, Month6, Month9, Month12, Month15, Month18, Month21 and Month24.

PD measurements will be performed at D1, M1, M3, M6, M9, M12, M15, M18, M21 and M24.

Compliance under cysteamine will be measured from D1 to M24 using electronic devices (date and time of bottle opening are recorded), accountability of study treatment and information of patients' diary. Compliance measurement between two consecutive study visits will be expressed as the proportion of the observed number of opening compared to the expected number and the cumulated dose taken compared to the expected dose.Compliance will be first considered as a dichotomous variable, the compliance being described as satisfactory if greater than 95% during a specific period.Compliance will then be considered as a quantitative variable

Secondary Outcome Measures
NameTimeMethod
Concentration of cysteamine in the CSF, associated to a measurement of the CSF pressureat any visit
Concentration effect, dose effect model.at month 6

pharmacokinetic profile and 2 timepoints pharmacodynamics.

Presence or absence and accountability of cystine crystals.Every 6 month : Month1, Month6, Month12, Month18, Month24

Eye examination

Memory and visuoperceptual tests repeated during the studyat Day 1 and two years
concentration of cysteamine in blood (measured by toxicological HPLC analysis with fluorescence detection)at Day1 and every 3 months : month1, month3, month6, month9, month12, month15, month18, month21, month24
presence or absence of cystine accumulation, determination of the sites of cystine accumulation in the CNS and relationship with the compliance to cysteamine treatment, relationship with neuropsychological status.at Month 1 and two years

NMRS assessments

discrimination of urine and blood samples,from metabolic spectroscopic data obtained by nuclear magnetic resonance.Every 3 months during 6 months and every 6 months to month 6 until two years

Perturbed metabolic network resulting from the intake of cysteamine in comparison to controls,using urine and blood samples available for patients with cystinosis and their controls

Trial Locations

Locations (1)

Hospices Civils de Lyon

🇫🇷

Lyon, France

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