How Does Dietary Carbohydrate Influence the Formation of an Atherogenic Lipoprotein Phenotype?
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 27
- 试验地点
- 2
- 主要终点
- Production Rate of VLDL-1 Triacylglycerol (TAG)
研究概览
简要总结
The hypothesis of this study is that a diet high in sugars will increase abnormalities in blood lipids which are associated with increased cardiovascular disease risk, relative to a diet which is low in sugar. We predict that this potentially adverse effect of dietary sugars on blood lipids will be more pronounced in people with a raised level of stored fat inside their liver, as compared to people with a low level of stored fat.
详细描述
This study aims to determine the metabolic mechanism(s) by which dietary extrinsic sugars (sucrose and fructose), promote the formation of a high risk dyslipidaemia, known as an atherogenic lipoprotein phenotype (raised plasma triglyceride, low HDL and predominance of small, dense LDL), in men with raised cardio-metabolic risk and percentage of liver fat, as determined by magnetic resonance spectroscopy (MRS). The study examined the impact of diets high and low in extrinsic sugars, on the metabolism of lipids and lipoproteins in vivo, of two groups of men with a high (>10%)and low (<2%)percentage of liver fat, by the trace-labelling of these lipid moieties with stable isotopes, and detection by gas chromatography mass spectrometry. The study had a two-way cross-over design, with two, 12 week dietary interventions separated by a six week wash-out period. The dietary intervention with high and low sugars was achieved by a dietary exchange with supermarket foods, which were consumed within the homes of the participants.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Male gender,
- •Increased cardio-metabolic risk ('RISCK' criteria Jebb et al (2010) Am J Clin Nutr 92, 748-758).
- •Apo E3E3 genotype
排除标准
- •Any abnormal result in blood screen (renal and liver function, haematology)
- •Excessive alcohol consumption (>27units/week)
- •Medication likely to affect lipid metabolism
- •>3kg weight loss in preceding 3 months
- •Any medical condition (eg. GI tract, allergies) affecting lipid metabolism or ability to comply with dietary interventions
- •Involvement in any other study
结局指标
主要结局
Production Rate of VLDL-1 Triacylglycerol (TAG)
时间窗: After (post-diet) two 12 week diets (high sugar versus low sugar) in men with NAFLD (n=11) versus Controls (n=14)
The in vivo production rate of VLDL-1 TAG, trace-labelled with \[1,1,2,3,3-H5\] glycerol, measured in units of grams/day.
Production Rate VLDL-1 Apoprotein B
时间窗: After (post-diet) two 12 week diets (high sugar versus low sugar) in men with NAFLD (n=11) versus Controls (n=14)
The in vivo production rate of VLDL-1 apoprotein B, trace-labelled with \[I-13C\] leucine (leucine with carbon-13), measured in units of milligrams/day.
次要结局
- De Novo Lipogenesis (Rate of Triacylglycerol (TAG) Synthesis in the Liver) as Measured by Contribution to VLDL-1 TAG Production Rate(After (post-diet) two 12 week diets (high sugar versus low sugar) in men with NAFLD (n=11) versus Controls (n=14))
- Intra-hepatocellular Lipid (IHCL) or % Liver Fat(After (post-diet) two 12 week diets (high sugar versus low sugar) in men with NAFLD (n=11) versus Controls (n=14))
- Kinetics of Systemic Non-esterified Fatty Acids by [C-13]-Trace-labelled Palmitate(After (post-diet) two 12 week diets (high sugar versus low sugar) in men with NAFLD (n=11) versus Controls (n=14))
- Plasma Concentration of Triacylglycerol(After (post-diet) two 12 week diets (high sugar versus low sugar) in men with NAFLD (n=11) versus Controls (n=14))
研究者
Bruce A. Griffin
Professor of Nutritional Metabolism
University of Surrey