Elucidation of the pathogenesis of chronic lower respiratory tract infection focusing on the phagocytic ability of lung macrophage phenotypes and the bacterial flora of the lower airway
Not Applicable
- Conditions
- chronic lower respiratory tract infection
- Registration Number
- JPRN-UMIN000033996
- Lead Sponsor
- Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan
- Brief Summary
This study revealed that AMs of the patients with NTM infection accounted for a higher rate of non-polarized (HLA-DR+CD40-CD163-) macrophage than that in the controls. In addition, AMs in the NTM group had an impaired ability to phagocytose S. aureus. In vitro experiments revealed increased intracellular S. aureus counts in M. intracellulare-infected macrophages compared with those in non-NTM-infected macrophages.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete: follow-up complete
- Sex
- All
- Target Recruitment
- 30
Inclusion Criteria
Not provided
Exclusion Criteria
The following patients are excluded. 1. Patients who do not agree with this study 2. Patients for whom bronchoscopy is difficult to preform because of comorbidity and who do not agree with bronchoscopy
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method We compare patients with and without pulmonary NTM disease and evaluate the statistically significant differences in the results of the bacterial flora analysis, the differentiation ability according to the macrophages phenotypes (proportion of macrophages phenotype), and the CT findings.
- Secondary Outcome Measures
Name Time Method We evaluate the following problems at the start of the test and 24 to 28 weeks after this trial started. 1) Clinical manifestations, laboratory findings 2) Microbiological evaluation: Transition of causative bacteria with sputum 3) Cytokines, oxidative stress markers in BALF (including TNF-a, NOS, HO-1, IL-4, IL-6, IL-8, IL-13 and others). 4) The time-dependent change of the CT findings