The Potential Association of Long Non-coding RNA (NEAT1 and MALAT1) in the Process of Epithelial-mesenchymal Transition in Periodontitis
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 发起方
- 入组人数
- 48
- 试验地点
- 1
- 主要终点
- determining the relative expression of lncRNAs (NEAT1 and MALAT1) in healthy and diseased periodontal tissue.
研究概览
简要总结
Periodontitis is a polymicrobial, inflammatory condition affecting tooth-supporting tissues. It is characterized by a progressive loss of epithelial attachment and resorption of alveolar bone, which can lead to tooth loss. Epithelial-mesenchymal transition (EMT) has been proposed as a reversible process that shifts the cell phenotype from epithelial to mesenchymal-like and may be involved in the process of periodontal inflammation either completely or partially. Long non-coding RNAs (lncRNAs) are a large class of regulatory transcripts longer than 200 nucleotides lacking evident protein-coding potential. Studies have shown that lncRNA dysregulation plays key roles in human diseases, including cancer, by modulating the epithelial-mesenchymal transition (EMT). The involvement of lncRNAs markers in the pathogenesis of periodontal disease induced EMT has not been studied thoroughly and have created a gap in the knowledge.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Patients with no history of systemic disease e.g., diabetes.
- •Both males and females will be included in the study.
- •Control group: patients fit the case definition of gingival health2, and confirmed by Histopathological inflammatory cell infiltration analysis.
- •Periodontitis group: patients fit the case definition of periodontal disease referred for periodontal flap surgery (modified Widman's flap).
- •Patients radiographically confirmed with infrabony pocket eligible for periodontal flap surgery.
排除标准
- •Patients not willing to participate or consent.
- •Patient taking medications that affect or modify immunity (e.g., corticosteroid), for the last 3 months.
- •Patient taking NSAIDs for the last 3 months.
- •Active smoker.
结局指标
主要结局
determining the relative expression of lncRNAs (NEAT1 and MALAT1) in healthy and diseased periodontal tissue.
时间窗: one year
reverse transcription real time polymerase chain reaction
次要结局
- determine the expression of EMT-related genes (E-cadherin, Vimentin, β-catenin, and Snail1), in healthy and diseased tissues, and their correlation with lncRNAs and clinical parameters.(one year)
研究者
Ali A Abdulkareem
assistant professor- department of periodontics-college of dentistry- Baghdad university
University of Baghdad