The Potential Association of Long Non-coding RNA (NEAT1 and MALAT1) in the Process of Epithelial-mesenchymal Transition in Periodontitis

Active, not recruiting

• Conditions: Epithelial Mesenchymal Transition; Long Noncoding RNA; Periodontal Disease, AVDC Stage 3and 4
• Interventions: Other: periodontitis challenge

• Registration Number: NCT05995067
• Lead Sponsor: Ali A Abdulkareem

Brief Summary

Periodontitis is a polymicrobial, inflammatory condition affecting tooth-supporting tissues. It is characterized by a progressive loss of epithelial attachment and resorption of alveolar bone, which can lead to tooth loss. Epithelial-mesenchymal transition (EMT) has been proposed as a reversible process that shifts the cell phenotype from epithelial to mesenchymal-like and may be involved in the process of periodontal inflammation either completely or partially. Long non-coding RNAs (lncRNAs) are a large class of regulatory transcripts longer than 200 nucleotides lacking evident protein-coding potential. Studies have shown that lncRNA dysregulation plays key roles in human diseases, including cancer, by modulating the epithelial-mesenchymal transition (EMT). The involvement of lncRNAs markers in the pathogenesis of periodontal disease induced EMT has not been studied thoroughly and have created a gap in the knowledge.

Detailed Description

Not available

Study Timeline

• Study Start: 2022-02-14
• Study First Submitted: 2023-08-08
• Study First Submitted QC: 2023-08-08
• Study First Posted: 2023-08-16
• Status Verified: 2023-12
• Last Update Submitted: 2023-12-09
• Last Update Posted: 2023-12-12
• Primary Completion: 2024-01-30
• Study Completion: 2024-01-30

Recruitment & Eligibility

• Status: ACTIVE_NOT_RECRUITING
• Sex: All
• Target Recruitment: 48

Inclusion Criteria

• Patients with no history of systemic disease e.g., diabetes.
• Both males and females will be included in the study.
• Control group: patients fit the case definition of gingival health2, and confirmed by Histopathological inflammatory cell infiltration analysis.
• Periodontitis group: patients fit the case definition of periodontal disease referred for periodontal flap surgery (modified Widman's flap).
• Patients radiographically confirmed with infrabony pocket eligible for periodontal flap surgery.

Exclusion Criteria

• Patients not willing to participate or consent.
• Patient taking medications that affect or modify immunity (e.g., corticosteroid), for the last 3 months.
• Patient taking NSAIDs for the last 3 months.
• Active smoker.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
study group	periodontitis challenge	-diseased group-/with the condition (periodontitis)

Primary Outcome Measures

Name	Time	Method
determining the relative expression of lncRNAs (NEAT1 and MALAT1) in healthy and diseased periodontal tissue.	one year	reverse transcription real time polymerase chain reaction

Secondary Outcome Measures

Name	Time	Method
determine the expression of EMT-related genes (E-cadherin, Vimentin, β-catenin, and Snail1), in healthy and diseased tissues, and their correlation with lncRNAs and clinical parameters.	one year	reverse transcription real time polymerase chain reaction

Trial Locations

Locations (1)

saif mohammed T AL Mufti; 🇮🇶 Baghdad, Iraq