Effect of Degranulation Needle With Different Diameters on the Rate Good Embryo in IVF

Not Applicable

• Conditions: Embryo
• Interventions: Device: 140 microns denuding pipette; Device: 150 microns denuding pipette

• Registration Number: NCT03810040
• Lead Sponsor: Tang-Du Hospital

Brief Summary

Good-quality embryos are critical for the success of in vitro fertilization (IVF). But, to date, there is no report of the effect of different diameters denuding pipette on the embryos quality. To investigate the effect of denuding pipette in IVF outcomes, we plan to recruit women undergoing IVF treatment cycles and classified them into two groups according to the different diameters. Consequently, the rate of fertilization, zygotes cleaved, top quality embryos on D3, and blastocysts obtained were recorded.

Detailed Description

Good-quality embryos are critical for the success of in vitro fertilization (IVF). But, to date, there is no report of the effect of different diameters denuding pipette on the embryos quality. To investigate the effect of denuding pipette in IVF outcomes, we plan to recruit women undergoing IVF treatment cycles and classified them into two groups according to the different diameters. The participants were divided into two group. Odd-numbered days the zygotes were degranulated with the 140 microns needles. Even-numbered days the zygotes were degranulated with the 150 microns needles. Consequently, the rate of fertilization, zygotes cleaved, top quality embryos on D3, and blastocysts obtained were recorded.

Study Timeline

• Study First Submitted: 2018-07-05
• Status Verified: 2019-01
• Study First Submitted QC: 2019-01-17
• Last Update Submitted: 2019-01-17
• Study First Posted: 2019-01-18
• Last Update Posted: 2019-01-18
• Study Start: 2019-02
• Primary Completion: 2019-05
• Study Completion: 2019-11

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Female
• Target Recruitment: 400

Inclusion Criteria

• first cyele of IVF treatment

Exclusion Criteria

• oocyte number less than 4
• oocyte number over than 25

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Odd-numbered days	140 microns denuding pipette	Once odd-numbered days the zytoges of IVF cycle were collected. The 140 microns denuding pipette was used.
Even-numbered days	150 microns denuding pipette	Once even-numbered days the zytoges of IVF cycle were collected. The 150 microns denuding pipette was used.

Primary Outcome Measures

Name	Time	Method
quality of embryo	one year	embryo quality assessed by good embryo rate

Secondary Outcome Measures

Name	Time	Method
clinical outcome - clinical prengancy rate	three years	clinical prengancy rate

Trial Locations

Locations (1)

Tangdu Hospital; 🇨🇳 Xi'an, Shaanxi, China