Cathelicidin LL-37 Levels in the Gingival Crevicular Fluid and the Saliva of Smokers and Non-smokers With Stage III,IV Periodontitis :An Observational Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- LL-37 levels in the GCF and saliva
研究概览
简要总结
Periodontitis is considered an inflammatory disease that results in the disruption of oral hemeostasis and is associated with the presence of dental plaque influenced by genetic and environmental factors. It is well-establised that smokers have an increased severity of periodontal disease,a higher prevalance of tooth loss and total edentulism.
详细描述
Antimicrobial peptides (AMPs) are soluble molecules participating in the innate immunity of mucosal surfaces against pathogens. Dysregulated expression of AMPs has been considered a possible causal mechanistic link in the microbial dysbiosis associated with periodontal disease and some risk factors, including tobacco smoking, diabetes mellitus, and obesity.Antimicrobial peptides as a part of innate immunity are important molecules that provide a balance between disease and health. Moreover, they are considered to be an important intermediate step in initiating an initial immune response against the microbial side of the gingival epithelium and in the transition to acquired immunity. Cathelicidins, in particular - which play an important role in regulating the immunological response of oral tissues, are a group of peptides released from neutrophil granules and they are present only in humans in the form of LL-37.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Presence of a minimum of 15 natural teeth.
- •At least four non-adjacent teeth sites in each jaw having CAL ≥ 5 mm and PD > 5 mm in one or more sites.
- •Bleeding on probing (BOP) ≥ 30%.
- •Tooth loss due to periodontitis ≤ 4 teeth.
- •For smoker's patients; study will include patients who regularly smoked at least 10 cigarettes/day for a minimum of 5 years.
- •For non-smokers patients; study will include patients who had never smoked.
排除标准
- •Individuals with a history of systemic diseases or immunological disorders.
- •Individuals using hormone replacement therapy.
- •Pregnant and lactating women.
- •Individuals that received periodontal treatment within the last 6 months.
- •Individuals with a history of systemic antibiotics and anti-inflammatory drugs within the last 3 months.
结局指标
主要结局
LL-37 levels in the GCF and saliva
时间窗: Through study completion,an average of 1 year
GCF,Saiva sampling to assess LL-37 levels by ELISA kit.
次要结局
未报告次要终点
研究者
Mai Magdy Abu El Eneen
principle investigator
Cairo University