Novel Approaches to Enhance Insulin-sensitizing Effects of Exercise: Targeting PAHSA Metabolism
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 60
- 试验地点
- 2
- 主要终点
- Assessment of PAHSA levels and its changes in response to exercise and fasting in cohorts differing in age and fat mass
研究概览
简要总结
Exercise represents an important tool in the prevention and treatment of metabolic disorders associated with obesity and aging, such as type 2 diabetes and cardiovascular disease. Besides skeletal muscle and its myokinins, the metabolic effects of exercise also rely on the induction of favorable changes in adipose tissue function. For example, adipose tissue is a source of lipokinins from the family of palmitic acid esters of hydroxy fatty acids (PAHSA), which have anti-inflammatory and insulin-sensitizing properties. We have recently shown that 4 months of exercise training increases PAHSA levels in adipose tissue and circulation. However, the mechanisms involved in the induction of PAHSA levels in response to exercise are unknown. The aim of the Effect of Acute Bout of Exercise on Levels of PAHSA (ETAPA) project is therefore to investigate the regulation of PAHSA metabolism in response to both acute and chronic exercise. To achieve this goal, we will employ state-of-the-art analytical methods to measure PAHSA levels in both adipose tissue and circulation of subjects of various ages and adiposity status. The main output of the ETAPA project will be the proof of principle regarding the important role of PAHSA lipokinins in exercise-induced enhancement of insulin sensitivity and the identification of potential drug targets that could be used to further improve PAHSA metabolism for the treatment of metabolic disorders associated with aging or obesity.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 25 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •healthy young lean, young obese and older omnivorous men and women as defined by BMI, self-reported activity, self-reported medical history and self-reported diet assessment
- •must be able to withstand repeated blood draws
- •must be able to undergo abdominal fat biopsy
排除标准
- •use of betablockers
- •use of glucocorticoids
- •use of non-steroidal anti-inflammatory drugs
- •use of metformin in prediabetes
- •use of psychiatric drugs such as selective serotonin reuptake inhibitors, serotonin and norepinephrine reuptake inhibitors, anticonvulsants and others
- •oncologic malignancy
- •chronic inflammatory or autoimmune diseases
- •diabetes mellitus
- •chronic ischemic heart disease
- •cardiovascular and pulmonary disease
- •renal and hepatological disease as assessed per biochemistry
- •musculo-skeletal deviations limiting physical performance
- •substance abuse
- •other than omnivorous diet
结局指标
主要结局
Assessment of PAHSA levels and its changes in response to exercise and fasting in cohorts differing in age and fat mass
时间窗: 2 years
PAHSA levels \[nmol/l\] in serum sampled at pre-set time-points during control and intervention visits (including exercise session or plain fasting) and in subcutaneous abdominal white adipose tissue will be assessed by quantitative targeted lipidomic analysis. Absolute and fold-change of PAHSA in plasma induced by experimental intervention (exercise, fasting) will be compared among individual cohorts.
次要结局
- Correlation of PAHSA levels with anthropometry and clinical characteristics(2 years)
- Correlation of PAHSA with various indexes of insulin sensitivity(2 years)
研究者
Lenka Rossmeislova
Principal Investigator, Head of Laboratory of Physiology and Pathophysiology of Adipose Tissue, Department of Pathophysiology, Third Faculty of Medicine
Charles University, Czech Republic